Abstract
Arcton 113 has proved effective in the removal of host membrane fragments which cosediment with influenza virus during differential centrifugation. The virus appeared morphologically undamaged by the procedure and retained its infectivity, neuraminidase, and hemagglutinating activity. Pyrogenicity, however, was considerably reduced.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allen R. J. The estimation of phosphorus. Biochem J. 1940 Jun;34(6):858–865. doi: 10.1042/bj0340858. [DOI] [PMC free article] [PubMed] [Google Scholar]
- BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
- EPSTEIN M. A. An investigation into the purifying effect of a fluorocarbon on vaccinia virus. Br J Exp Pathol. 1958 Aug;39(4):436–446. [PMC free article] [PubMed] [Google Scholar]
- HAMPARIAN V. V., MULLER F., HUMMELER K. Elimination of nonspecific components from viral antigens by fluorocarbon. J Immunol. 1958 Jun;80(6):468–475. [PubMed] [Google Scholar]
- Kaplan C., Turner G. S. Removal of encephalitogenicity from extracts of normal rabbit central nervous system by treatment with fluorocarbon. Nature. 1968 Aug 3;219(5153):445–446. doi: 10.1038/219445a0. [DOI] [PubMed] [Google Scholar]
- Kilbourne E. D., Schulman J. L., Schild G. C., Schloer G., Swanson J., Bucher D. Related studies of a recombinant influenza-virus vaccine. I. Derivation and characterization of virus and vaccine. J Infect Dis. 1971 Nov;124(5):449–462. doi: 10.1093/infdis/124.5.449. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- MANSON L. A., ROTHSTEIN E. L., RAKE G. W. Purification of poliovirus with fluorocarbon. Science. 1957 Mar 22;125(3247):546–547. doi: 10.1126/science.125.3247.546-a. [DOI] [PubMed] [Google Scholar]
- Reimer C. B., Baker R. S., Van Frank R. M., Newlin T. E., Cline G. B., Anderson N. G. Purification of large quantities of influenza virus by density gradient centrifugation. J Virol. 1967 Dec;1(6):1207–1216. doi: 10.1128/jvi.1.6.1207-1216.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- SIEGERT R., BRAUNE P. THE PYROGENS OF MYXOVIRUSES. II. RESISTANCE OF INFLUENZA A PYROGENS TO HEAT, ULTRAVIOLET, AND CHEMICAL TREATMENT. Virology. 1964 Oct;24:218–224. doi: 10.1016/0042-6822(64)90107-2. [DOI] [PubMed] [Google Scholar]
- WARREN L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959 Aug;234(8):1971–1975. [PubMed] [Google Scholar]

