Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1976 Jun;3(6):566–575. doi: 10.1128/jcm.3.6.566-575.1976

Rapid method for identification of gram-negative, nonfermentative bacilli.

L A Otto, M J Pickett
PMCID: PMC274357  PMID: 780371

Abstract

A rapid system (OA), based on oxidative attack of substrates, was developed for identification of gram-negative, nonfermentative bacillia (NFB). One hundred and twelve strains of NFB from 25 species (representing the genera Pseudomonas, Alcaligenes, Acinetobacter, Bordetella, Flavobacterium, Moraxella, and Xanthomonas) were assayed by OA, buffered single substrate, and oxidative/fermentative methods. The 38 substrates consisted of salts of organic acids, nitrogen-containing compounds, alcohols, and carbohydrates. Ninety-four percent of the test strains were identified by the OA method in 24 h, and 99% were identifiable in 48 h. Reproducibility was 99%. Correlation with buffered single substrate was 98% (all substrates) and 90% with the oxidative/fermentative method (carbohydrates only). Biochemical profiles of all strains are presented, as well as tables showing the most useful tests for identification.

Full text

PDF
566

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ballard R. W., Doudoroff M., Stanier R. Y., Mandel M. Taxonomy of the aerobic psuedomonads: Pseudomonas diminuta and P. vesiculare. J Gen Microbiol. 1968 Oct;53(3):349–361. doi: 10.1099/00221287-53-3-349. [DOI] [PubMed] [Google Scholar]
  2. Ballard R. W., Palleroni N. J., Doudoroff M., Stanier R. Y., Mandel M. Taxonomy of the aerobic pseudomonads: Pseudomonas cepacia, P. marginata, P. alliicola and P. caryophylli. J Gen Microbiol. 1970 Feb;60(2):199–214. doi: 10.1099/00221287-60-2-199. [DOI] [PubMed] [Google Scholar]
  3. Baumann P., Doudoroff M., Stanier R. Y. A study of the Moraxella group. II. Oxidative-negative species (genus Acinetobacter). J Bacteriol. 1968 May;95(5):1520–1541. doi: 10.1128/jb.95.5.1520-1541.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Baumann P., Doudoroff M., Stanier R. Y. Study of the Moraxella group. I. Genus Moraxella and the Neisseria catarrhalis group. J Bacteriol. 1968 Jan;95(1):58–73. doi: 10.1128/jb.95.1.58-73.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blazevic D. J., Koepcke M. H., Matsen J. M. Incidence and identification of Pseudomonas fluorescens and Pseudomonas putida in the clinical laboratory. Appl Microbiol. 1973 Jan;25(1):107–110. doi: 10.1128/am.25.1.107-110.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Carson L. A., Favero M. S., Bond W. W., Petersen N. J. Morphological, biochemical, and growth characteristics of pseudomonas cepacia from distilled water. Appl Microbiol. 1973 Mar;25(3):476–483. doi: 10.1128/am.25.3.476-483.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Chadwick P., Delisle G. J., Byer M. Biochemical identification of hospital enterobacteria by replica agar plating. Can J Microbiol. 1974 Dec;20(12):1653–1664. doi: 10.1139/m74-257. [DOI] [PubMed] [Google Scholar]
  8. Favero M. S., Carson L. A., Bond W. W., Petersen N. J. Pseudomonas aeruginosa: growth in distilled water from hospitals. Science. 1971 Aug 27;173(3999):836–838. doi: 10.1126/science.173.3999.836. [DOI] [PubMed] [Google Scholar]
  9. Gardner P., Griffin W. B., Swartz M. N., Kunz L. J. Nonfermentative gram-negative bacilli of nosocomial interest. Am J Med. 1970 Jun;48(6):735–749. doi: 10.1016/s0002-9343(70)80009-2. [DOI] [PubMed] [Google Scholar]
  10. Gilardi G. L. Characterization of Pseudomonas species isolated from clinical specimens. Appl Microbiol. 1971 Mar;21(3):414–419. doi: 10.1128/am.21.3.414-419.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gilardi G. L. Characterization of nonfermentative nonfastidious gram negative bacteria encountered in medical bacteriology. J Appl Bacteriol. 1971 Sep;34(3):623–644. doi: 10.1111/j.1365-2672.1971.tb02326.x. [DOI] [PubMed] [Google Scholar]
  12. Gilardi G. L. Diagnostic criteria for differentiation of pseudomonads pathogenic for man. Appl Microbiol. 1968 Oct;16(10):1497–1502. doi: 10.1128/am.16.10.1497-1502.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Gilardi G. L. Nonfermentative gram-negative bacteria encountered in clinical specimens. Antonie Van Leeuwenhoek. 1973;39(2):229–242. doi: 10.1007/BF02578855. [DOI] [PubMed] [Google Scholar]
  14. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Hardy P. C., Ederer G. M., Matsen J. M. Contamination of commercially packaged urinary catheter kits with the pseudomonad EO-1. N Engl J Med. 1970 Jan 1;282(1):33–35. doi: 10.1056/NEJM197001012820108. [DOI] [PubMed] [Google Scholar]
  16. Henriksen S. D. Moraxella, Acinetobacter, and the Mimeae. Bacteriol Rev. 1973 Dec;37(4):522–561. doi: 10.1128/br.37.4.522-561.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Juffs H. S. Identification of Pseudomonas spp. isolated from milk produced in South Eastern Queensland. J Appl Bacteriol. 1973 Dec;36(4):585–598. doi: 10.1111/j.1365-2672.1973.tb04145.x. [DOI] [PubMed] [Google Scholar]
  18. Palleroni N. J., Doudoroff M., Stanier R. Y., Solánes R. E., Mandel M. Taxonomy of the aerobic pseudomonads: the properties of the Pseudomonas stutzeri group. J Gen Microbiol. 1970 Feb;60(2):215–231. doi: 10.1099/00221287-60-2-215. [DOI] [PubMed] [Google Scholar]
  19. Pickett M. J., Pedersen M. M. Characterization of saccharolytic nonfermentative bacteria associated with man. Can J Microbiol. 1970 May;16(5):351–362. doi: 10.1139/m70-062. [DOI] [PubMed] [Google Scholar]
  20. Pickett M. J., Pedersen M. M. Nonfermentative bacilli associated with man. II. Detection and identification. Am J Clin Pathol. 1970 Aug;54(2):164–177. doi: 10.1093/ajcp/54.2.164. [DOI] [PubMed] [Google Scholar]
  21. Pickett M. J., Pedersen M. M. Salient features of nonsaccharolytic and weakly saccharolytic nonfermentative rods. Can J Microbiol. 1970 Jun;16(6):401–409. doi: 10.1139/m70-069. [DOI] [PubMed] [Google Scholar]
  22. Rosenthal S. L. A simplified method for single carbon source tests with Pseudomonas species. J Appl Bacteriol. 1974 Sep;37(3):437–441. doi: 10.1111/j.1365-2672.1974.tb00459.x. [DOI] [PubMed] [Google Scholar]
  23. Snell J. J., Lapage S. P. Comparison of four methods for demonstrating glucose breakdown by bacteria. J Gen Microbiol. 1971 Oct;68(2):221–225. doi: 10.1099/00221287-68-2-221. [DOI] [PubMed] [Google Scholar]
  24. Stanier R. Y., Palleroni N. J., Doudoroff M. The aerobic pseudomonads: a taxonomic study. J Gen Microbiol. 1966 May;43(2):159–271. doi: 10.1099/00221287-43-2-159. [DOI] [PubMed] [Google Scholar]
  25. Sutter V. L. Identification of Pseudomonas species isolated from hospital environment and human sources. Appl Microbiol. 1968 Oct;16(10):1532–1538. doi: 10.1128/am.16.10.1532-1538.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES