Abstract
Three bacteriological techniques for the isolation of group B streptococci in vaginal cultures were compared. A selective broth medium (SBM) containing gentamicin and nalidixic acid was more sensitive for the detection of vaginal isolates (28/76, 36.8%) from 76 women enrolled in a venereal disease clinic than was an identical selective plate medium (SPM) (17/76, 25%). Similarly, SBM allowed identification of positive cultures from college women (82/459, 17.9%) significantly more often than direct inoculation of swabs onto nonselective blood agar medium (43/460, 9.4%; chi2 = 42.2, P = less than 0.001). Failure to isolate group B streptococci detected in SBM occurred in 32.1% cultures by SPM and 49.4% of cultures by nonselective agar medium. Multiple serotypes were detected in a single vaginal culture from approximately 5% of the patients studied. These data support the routine use of SBM for the most accurate identification of women vaginally colonized with group B Streptococcus.
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Selected References
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- Baker C. J., Barrett F. F. Transmission of group B streptococci among parturient women and their neonates. J Pediatr. 1973 Dec;83(6):919–925. doi: 10.1016/s0022-3476(73)80524-4. [DOI] [PubMed] [Google Scholar]
- Baker C. J., Clark D. J., Barrett F. F. Selective broth medium for isolation of group B streptococci. Appl Microbiol. 1973 Dec;26(6):884–885. doi: 10.1128/am.26.6.884-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Franciosi R. A., Knostman J. D., Zimmerman R. A. Group B streptococcal neonatal and infant infections. J Pediatr. 1973 Apr;82(4):707–718. doi: 10.1016/s0022-3476(73)80604-3. [DOI] [PubMed] [Google Scholar]
- Howard J. B., McCracken G. H., Jr The spectrum of group B streptococcal infections in infancy. Am J Dis Child. 1974 Dec;128(6):815–818. doi: 10.1001/archpedi.1974.02110310063011. [DOI] [PubMed] [Google Scholar]
- Steere A. C., Aber R. C., Warford L. R., Murphy K. E., Feeley J. C., Hayes P. S., Wilkinson H. W., Facklam R. R. Possible nosocomial transmission of group B streptococci in a newborn nursery. J Pediatr. 1975 Nov;87(5):784–787. doi: 10.1016/s0022-3476(75)80311-8. [DOI] [PubMed] [Google Scholar]