Abstract
In this study we utilized the salt-tolerant characteristics of vibrios to develop a more selective medium by addition of NaCl to thiosulfate citrate bile salts sucrose (TCBS) agar. The effect of adding salt to TCBS agar varied greatly among brands of TCBS agar and between lots of the same brand. The addition of salt at concentrations as high as 1.5% (2.5% total NaCl) caused the inhibition of growth of three species of commonly encountered normal bowel flora and one strain of classical Vibrio cholerae but did not compromise the use of TCBS agar for isolation of V. cholerae biotype El Tor.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Baine W. B., Mazzotti M., Greco D., Izzo E., Zampieri A., Angioni G., Di Gioia M., Gangarosa E. J., Pocchiari F. Epidemiology of cholera in Italy in 1973. Lancet. 1974 Dec 7;2(7893):1370–1374. doi: 10.1016/s0140-6736(74)92233-8. [DOI] [PubMed] [Google Scholar]
- De Lorenzo F., Soscia M., Manzillo G., Balestrieri G. G. Epidemic of cholera El Tor in Naples, 1973. Lancet. 1974 Apr;1(7859):669–669. doi: 10.1016/s0140-6736(74)93214-0. [DOI] [PubMed] [Google Scholar]
- McCormack W. M., DeWitt W. E., Bailey P. E., Morris G. K., Soeharjono P., Gangarosa E. J. Evaluation of thiosulfate-citrate-bile salts-sucrose agar, a selective medium for the isolation of Vibrio cholerae and other pathogenic vibrios. J Infect Dis. 1974 May;129(5):497–500. doi: 10.1093/infdis/129.5.497. [DOI] [PubMed] [Google Scholar]
- Molenda J. R., Johnson W. G., Fishbein M., Wentz B., Mehlman I. J., Dadisman T. A., Jr Vibrio parahaemolyticus gastroenteritis in Maryland: laboratory aspects. Appl Microbiol. 1972 Sep;24(3):444–448. doi: 10.1128/am.24.3.444-448.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Weissman J. B., DeWitt W. E., Thompson J., Muchnick C. N., Portnoy B. L., Feeley J. C., Gangarosa E. J. A case of cholera in Texas, 1973. Am J Epidemiol. 1974 Dec;100(6):487–498. doi: 10.1093/oxfordjournals.aje.a112061. [DOI] [PubMed] [Google Scholar]