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. 1976 Sep;4(3):216–224. doi: 10.1128/jcm.4.3.216-224.1976

Critical analysis of hypertonic medium and agitation in detection of bacteremia.

P D Ellner, T E Kiehn, J L Beebe, L R McCarthy
PMCID: PMC274439  PMID: 972189

Abstract

Over 18,000 clinical specimens collected in Vacutainer tubes with sodium polyanethol sulfonate were inoculated into modified Columbia broth (MCB) with and without 10% sucrose. The effects of venting and shaking on recovery were studied. The volume of the blood had a definite effect on the recovery rate. When inoculum size was held constant, recovery of aerobic and facultative organisms was maximal in vented and shaken bottles; the presence of sucrose had no demonstrable effect, recovery of anaerobes was maximal using an unvented bottle incubated under stationary conditions; a significantly greater recovery of facultatives and a marginally greater recovery of anaerobes was obtained with the hypertonic formulation. We conclude that a hypertonic formulation of MCB offers no advantage in the recovery of anaerobes but is of value in the recovery of facultatives and anaerobes. It is recommended that blood cultures be routinely inoculated into isotonic MCB and then vented and shaken for at least 4 hours, and hypertonic MCB incubated without venting or shaking.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blazevic D. J., Stemper J. E., Matsen J. M. Effect of aerobic and anaerobic atmospheres on isolation of organisms from blood cultures. J Clin Microbiol. 1975 Feb;1(2):154–156. doi: 10.1128/jcm.1.2.154-156.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. COCHRAN W. G. The comparison of percentages in matched samples. Biometrika. 1950 Dec;37(3-4):256–266. [PubMed] [Google Scholar]
  3. Caslow M., Ellner P. D., Kiehn T. E. Comparison of the BACTEC system with blind subculture for the detection of bacteremia. Appl Microbiol. 1974 Sep;28(3):435–438. doi: 10.1128/am.28.3.435-438.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ellner P. D., Granato P. A., May C. B. Recovery and identification of anaerobes: a system suitable for the routine clinical laboratory. Appl Microbiol. 1973 Dec;26(6):904–913. doi: 10.1128/am.26.6.904-913.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ellner P. D. System for inoculation of blood in the laboratory. Appl Microbiol. 1968 Dec;16(12):1892–1894. doi: 10.1128/am.16.12.1892-1894.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gantz N. M., Medeiros A. A., Swain J. L., O'Brien T. F. Vacuum blood-culture bottles inhibiting growth of Candida and fostering growth of Bacteroides. Lancet. 1974 Nov 16;2(7890):1174–1176. doi: 10.1016/s0140-6736(74)90813-7. [DOI] [PubMed] [Google Scholar]
  7. Harkness J. L., Hall M., Ilstrup D. M., Washington J. A., 2nd Effects of atmosphere of incubation and of routine subcultures on detection of bacteremia in vacuum blood culture bottles. J Clin Microbiol. 1975 Oct;2(4):296–299. doi: 10.1128/jcm.2.4.296-299.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Henrichsen J., Bruun B. An evaluation of the effects of a high concentration of sucrose in blood culture media. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Dec;81(6):707–710. doi: 10.1111/j.1699-0463.1973.tb02264.x. [DOI] [PubMed] [Google Scholar]
  9. KAGAN C. Y., MIKHAILOVA V. S. ISOLATION OF L-FORMS OF STREPTOCOCCI FROM THE BLOOD OF PATIENTS WITH RHEUMATISM AND ENDOCARDITIS. J Hyg Epidemiol Microbiol Immunol. 1963;7:327–343. [PubMed] [Google Scholar]
  10. Louria D. B., Kaminski T., Grieco M., Singer J. Aberrant forms of bacteria and fungi found in blood or cerebrospinal fluid. Arch Intern Med. 1969 Jul;124(1):39–48. [PubMed] [Google Scholar]
  11. MATTMAN L. H., MATTMAN P. E. L FORMS OF STREPTOCOCCUS FECALIS IN SEPTICEMIA. Arch Intern Med. 1965 Mar;115:315–321. doi: 10.1001/archinte.1965.03860150059011. [DOI] [PubMed] [Google Scholar]
  12. Morello J. A., Ellner P. D. New medium for blood cultures. Appl Microbiol. 1969 Jan;17(1):68–70. doi: 10.1128/am.17.1.68-70.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Muschel L. H., Larsen L. J. Effect of hypertonic sucrose upon the immune bactericidal reaction. Infect Immun. 1970 Jan;1(1):51–55. doi: 10.1128/iai.1.1.51-55.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Neu H. C., Goldreyer B. Isolation of protoplasts in a case of enterococcal endocarditis. Am J Med. 1968 Nov;45(5):784–788. doi: 10.1016/0002-9343(68)90211-8. [DOI] [PubMed] [Google Scholar]
  15. Rosner R. A quantitative evaluation of three blood culture systems. Am J Clin Pathol. 1972 Feb;57(2):220–227. doi: 10.1093/ajcp/57.2.220. [DOI] [PubMed] [Google Scholar]
  16. Rosner R. Comparison of a blood culture system containing liquoid and sucrose with systems containing either reagent alone. Appl Microbiol. 1970 Feb;19(2):281–282. doi: 10.1128/am.19.2.281-282.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rosner R. Comparison of macroscopic, microscopic, and radiometric examinations of clinical blood cultures in hypertonic media. Appl Microbiol. 1974 Oct;28(4):644–646. doi: 10.1128/am.28.4.644-646.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rosner R. Evaluation of four blood culture systems using parallel culture methods. Appl Microbiol. 1974 Aug;28(2):245–247. doi: 10.1128/am.28.2.245-247.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rosner R. Isolation of Candida protoplasts from a case of Candida endocarditis. J Bacteriol. 1966 Mar;91(3):1320–1326. doi: 10.1128/jb.91.3.1320-1326.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sullivan N. M., Sutter V. L., Carter W. T., Attebery H. R., Finegold S. M. Bacteremia after genitourinary tract manipulation: bacteriological aspects and evaluation of various blood culture systems. Appl Microbiol. 1972 Jun;23(6):1101–1106. doi: 10.1128/am.23.6.1101-1106.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Todd J. K., Roe M. H. Rapid detection of bacteremia by an early subculture technic. Am J Clin Pathol. 1975 Nov;64(5):694–699. doi: 10.1093/ajcp/64.5.694. [DOI] [PubMed] [Google Scholar]
  22. Washington J. A., 2nd Comparison of two commercially available media for detection of bacteremia. Appl Microbiol. 1971 Oct;22(4):604–607. doi: 10.1128/am.22.4.604-607.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Washington J. A., 2nd, Hall M. M., Warren E. Evaluation of blood culture media supplemented with sucrose or with cysteine. J Clin Microbiol. 1975 Jan;1(1):79–81. doi: 10.1128/jcm.1.1.79-81.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wasilauskas B. L., Ellner P. D. Presumptive identification of bacteria from blood cultures in four hours. J Infect Dis. 1971 Nov;124(5):499–504. doi: 10.1093/infdis/124.5.499. [DOI] [PubMed] [Google Scholar]

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