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. 1976 Sep;4(3):266–269. doi: 10.1128/jcm.4.3.266-269.1976

Primary isolation of Mycobacterium avium complex-serotype 6 on blood agar.

J E Thigpen, V L Thierry, B N Gupta
PMCID: PMC274448  PMID: 972192

Abstract

Mycobacterium avium complex-serotype 6 was isolated in pure culture on blood agar plates from inocula taken from the heart blood, lungs, liver, kidneys, and spleen of a naturally infected captive female opossum (Didelphis marsupialis virginiana). Repeat cultures from stored tissues and transfer of colonies from original blood agar plates revealed that the mycobacterium grew on tryptose, brilliant green, eosin-methylene blue. Sabouraud glucose, and mycobiotic agar plates and in Fletcher leptospira medium. The cultural, biochemical, and serological characteristics of the test isolate were compared with other mycobacteria. This is the first report to describe the primary isolation of a serotype from the M. avium complex from an animal species on blood agar or in Fletcher broth. In addition, this is the second documented report describing the isolation and identification of a mycobacterial species from the American opossum.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Borghans J. G., Stanford J. L. Mycobacterium chelonei in abscesses after injection of diphtheria-pertussis-tetanus-polio vaccine. Am Rev Respir Dis. 1973 Jan;107(1):1–8. doi: 10.1164/arrd.1973.107.1.1. [DOI] [PubMed] [Google Scholar]
  2. Brashear R. E., Lampton L. M. Atypical mycobacteria: a factor to consider in lung disease. Postgrad Med. 1975 Oct;58(4):117–121. doi: 10.1080/00325481.1975.11714173. [DOI] [PubMed] [Google Scholar]
  3. Crowley J., Liu P. I., Glassman A. B. Primary isolation of Mycobacterium chelonei subspecies abscessus from pus inoculated into peptone broth. Appl Microbiol. 1974 Dec;28(6):943–945. doi: 10.1128/am.28.6.943-945.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dubina J., Sula L., Kubín M., Vareková J. Incidence of M. avium and M. intracellulare in cattle and pigs. J Hyg Epidemiol Microbiol Immunol. 1974;18(1):15–21. [PubMed] [Google Scholar]
  5. Ferguson S. H., Wallace L. J., Dunbar F., Cacciatore R. Mycobacterium intracellulare (Battey bacillus) infection in a Florida wood duck (Aix sponsa). Some epidemiologic considerations. Am Rev Respir Dis. 1969 Dec;100(6):876–879. doi: 10.1164/arrd.1969.100.6.876. [DOI] [PubMed] [Google Scholar]
  6. Fogan L. Atypical mycobacteria. Their clinical, laboratory, and epidemiologic significance. Medicine (Baltimore) 1970 May;49(3):243–255. [PubMed] [Google Scholar]
  7. Kubica G. P. Differential identification of mycobacteria. VII. Key features for identification of clinically significant mycobacteria. Am Rev Respir Dis. 1973 Jan;107(1):9–21. doi: 10.1164/arrd.1973.107.1.9. [DOI] [PubMed] [Google Scholar]
  8. Kubica G. P., Gross W. M., Hawkins J. E., Sommers H. M., Vestal A. L., Wayne L. G. Laboratory services for mycobacterial diseases. Am Rev Respir Dis. 1975 Dec;112(6):773–787. doi: 10.1164/arrd.1975.112.6.773. [DOI] [PubMed] [Google Scholar]
  9. MOORE M., FRERICHS J. B. An unusual acid-fast infection of the knee with subcutaneous, abscess-like lesions of the gluteal region; report of a case with a study of the organism, Mycobacterium abscessus, n. sp. J Invest Dermatol. 1953 Feb;20(2):133–169. doi: 10.1038/jid.1953.18. [DOI] [PubMed] [Google Scholar]
  10. Moore T. D., Allen A. M., Ganaway J. R., Sevy C. E. A fatal infection in the opossum due to Mycobacterium intracellulare. J Infect Dis. 1971 Jun;123(6):569–578. doi: 10.1093/infdis/123.6.569. [DOI] [PubMed] [Google Scholar]
  11. RUNYON E. H. Anonymous mycobacteria in pulmonary disease. Med Clin North Am. 1959 Jan;43(1):273–290. doi: 10.1016/s0025-7125(16)34193-1. [DOI] [PubMed] [Google Scholar]
  12. Schaefer W. B. Incidence of the serotypes of Mycobacerium avium and atypical mycobacteria in human and animal diseases. Am Rev Respir Dis. 1968 Jan;97(1):18–23. doi: 10.1164/arrd.1968.97.1.18. [DOI] [PubMed] [Google Scholar]
  13. Schaefer W. B. Serologic identification and classification of the atypical mycobacteria by their agglutination. Am Rev Respir Dis. 1965 Dec;92(6):85–93. doi: 10.1164/arrd.1965.92.6P2.85. [DOI] [PubMed] [Google Scholar]
  14. Thoen C. O., Jarnagin J. L., Richards W. D. Isolation and identification of mycobacteria from porcine tissues: a three-year summary. Am J Vet Res. 1975 Sep;36(9):1383–1386. [PubMed] [Google Scholar]

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