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. 1976 Dec;4(6):511–514. doi: 10.1128/jcm.4.6.511-514.1976

Simplified scheme for identification of prompt lactose-fermenting members of the Enterobacteriaceae.

M J Hicks, K J Ryan
PMCID: PMC274512  PMID: 794078

Abstract

A brief, simplified scheme involving the spot indole test and colonial morphology was evaluated for genus level identification of prompt lactose-fermenting (PLF) members of the Enterobacteriaceae. One hundred and ninety-four consecutive, clinically important PLF gram-negative rods isolated in a clinical microbiology laboratory were identified by this simplified scheme, as well as by standard biochemical tests, and the API 20E (Analytab Products, Inc., Plainview, N.Y.) system. In the simplified scheme a flat, spot indole-positive colony was identified as Escherichia coli. Spot indole-negative organisms forming nucoid colonies were identified as Klebsiella sp. or Enterobacter sp. on the basis of semisolid motility and ornithine decarboxylase tests. Approximately 94% of the study isolates followed reactions typical for E. coli, Klebsiella sp., and Enterobacter sp. as defined by this simplified scheme. When compared with the standard and Analytab Products Inc. identifications, the overall accuracy was 97.4%. The accuracy of identification of E. coli, Klebsiella sp., and Enterobacter sp. was 98.1%, 95.6%, and 87.5%, respectively. This simplified scheme is recommended for identification of selected PLF isolates in the clinical microbiology laboratory.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blazevic D. J. Improved motility-indole medium. Appl Microbiol. 1968 Apr;16(4):668–668. doi: 10.1128/am.16.4.668-.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Blazevic D. J., Schreckenberger P. C., Matsen J. M. Evaluation of the PathoTec "Rapid I-D System". Appl Microbiol. 1973 Dec;26(6):886–889. doi: 10.1128/am.26.6.886-889.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Closs O., Digranes A. Rapid identification of prompt lactose-fermenting genera within the familyh Enterobacteriaceae. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;79(5):673–678. doi: 10.1111/j.1699-0463.1971.tb00095.x. [DOI] [PubMed] [Google Scholar]
  4. Finkelstein R. A., Punyashthiti K. Colonial recognition, a "new" approach for rapid diagnostic enteric bacteriology. J Bacteriol. 1967 Jun;93(6):1897–1905. doi: 10.1128/jb.93.6.1897-1905.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Goldin M. A comparison of multiple-test systems for the presumptive identification of enterobacteriaceae. Am J Med Technol. 1972 Aug;38(8):288–291. [PubMed] [Google Scholar]
  6. Hansen S. L., Hardesty D. R., Myers B. M. Evaluation of the BBL Minitek system for the identification of Enterobacteriaceae. Appl Microbiol. 1974 Nov;28(5):798–801. doi: 10.1128/am.28.5.798-801.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Isenberg H. D., Painter B. G. Comparison of conventional methods, the R-B system, and modified R-B system as guides to the major divisions of Enterobacteriaceae. Appl Microbiol. 1971 Dec;22(6):1126–1134. doi: 10.1128/am.22.6.1126-1134.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Isenberg H. D., Smith P. B., Balows A., Painter B. G., Rhoden D. L., Tomfohrde K. r-b Expanders: their use in identifying routinely and unusually reacting members of Enterobacteriaceae. Appl Microbiol. 1974 Mar;27(3):575–583. doi: 10.1128/am.27.3.575-583.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kiehn T. E., Brennan K., Ellner P. D. Evaluation of the Minitek system for identification of Enterobacteriaceae. Appl Microbiol. 1974 Oct;28(4):668–671. doi: 10.1128/am.28.4.668-671.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lassen J. Rapid identification of gram-negative rods using a three-tube method combined with a dichotomic key. Acta Pathol Microbiol Scand Suppl. 1975 Dec;83(6):525–533. doi: 10.1111/j.1699-0463.1975.tb00134.x. [DOI] [PubMed] [Google Scholar]
  11. Painter B. G., Isenberg H. D. Clinical laboratory experience with the improved Enterotube. Appl Microbiol. 1973 Jun;25(6):896–899. doi: 10.1128/am.25.6.896-899.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Reller L. B., Mirrett S. Motility-indole-lysine medium for presumptive identification of enteric pathogens of Enterobacteriaceae. J Clin Microbiol. 1975 Sep;2(3):247–252. doi: 10.1128/jcm.2.3.247-252.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rhoden D. L., Tomfohrde K. M., Smith P. B., Balows A. Evaluation of the improved Auxotab 1 System for identifying Enterobacteriaceae. Appl Microbiol. 1973 Aug;26(2):215–216. doi: 10.1128/am.26.2.215-216.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Robertson E. A., MacLowry J. D. Construction of an interpretive pattern directory for the API 10 S kit and analysis of its diagnostic accuracy. J Clin Microbiol. 1975 Jun;1(6):515–520. doi: 10.1128/jcm.1.6.515-520.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rosner R. Evaluation of the PathoTec "Rapid I-D System" and two additional Experimental reagent-impregnated paper strips. Appl Microbiol. 1973 Dec;26(6):890–893. doi: 10.1128/am.26.6.890-893.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Shayegani M., Hubbard M. E., Hiscott T., McGlynn D. Evaluation of the R/B and Minitek systems for identification of Enterobacteriaceae. J Clin Microbiol. 1975 Jun;1(6):504–508. doi: 10.1128/jcm.1.6.504-508.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith P. B., Rhoden D. L., Tomfohrde K. M. Evaluation of the pathotec Rapid I-D system for identification of Enterobacteriaceae. J Clin Microbiol. 1975 Apr;1(4):359–362. doi: 10.1128/jcm.1.4.359-362.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smith P. B., Tomfohrde K. M., Rhoden D. L., Balows A. API system: a multitube micromethod for identification of Enterobacteriaceae. Appl Microbiol. 1972 Sep;24(3):449–452. doi: 10.1128/am.24.3.449-452.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tomfohrde K. M., Rhoden D. L., Smith P. B., Balows A. Evaluation of the redesigned enterotube--a system for the identification of Enterobacteriaceae. Appl Microbiol. 1973 Feb;25(2):301–304. doi: 10.1128/am.25.2.301-304.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Washington J. A., 2nd, Yu P. K., Martin W. J. Evaluation of accuracy of multitest micromethod system for identification of Enterobacteriaceae. Appl Microbiol. 1971 Sep;22(3):267–269. doi: 10.1128/am.22.3.267-269.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wolfe M. W., Amsterdam D. New diagnostic system for the identification of lactose-fermenting gram-negative rods. Appl Microbiol. 1968 Oct;16(10):1528–1531. doi: 10.1128/am.16.10.1528-1531.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

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