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. Author manuscript; available in PMC: 2010 Sep 1.
Published in final edited form as: Biochimie. 2009 Jul 10;91(9):1072–1080. doi: 10.1016/j.biochi.2009.07.003

Fig. 3. Determination of kinetic characteristics of human ω-amidase/Nit2.

Fig. 3

Enzyme (0.82 μg) was incubated with varying concentrations of αKGM in 100 mM Tris-HCl buffer (pH 8.5) at 37°C. α-Ketoglutarate was measured as its 2,4-dinitrophenylhydrazone. Each point is the average ± s.d., N=3.