Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1977 Jan;5(1):9–14. doi: 10.1128/jcm.5.1.9-14.1977

Simplified complete medium for the growth of Neisseria gonorrhoeae.

R T Jones, R S Talley
PMCID: PMC274522  PMID: 401832

Abstract

A complete medium for the growth of Neisseria gonorrhoeae has been developed, using the same basic ingredients contained in commercial media but with fewer supplements. Based on a comparison of plating efficiencies, this medium (designated TTU Complete Medium) supported the growth of laboratory strains of N. gonorrhoeae equal to that obtained on commercial GC agar base medium supplemented with Iso VitaleX. It was also equivalent to the commercial medium in supporting the growth of 67 clinical isolates and 6 auxotypes of N. gonorrhoeae when tested by the streak plate method. Based on turbidity measurements and viable plate counts, the liquid equivalent of TTU Complete Medium supported maximal growth yields of N. gonorrhoeae. The toxicity of different brands of agar appeared to be a major factor in preventing the growth of N. gonorrhoeae strains on solid media. The addition of starch neutralized the toxicity of some types of agar but not of others.

Full text

PDF
9

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amies C. R., Garabedian M. An easily prepared selective medium for the cultivation of Neisseria gonorrhoeae. Br J Vener Dis. 1967 Jun;43(2):137–139. doi: 10.1136/sti.43.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CURTIS S. R., 3rd CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI. J Bacteriol. 1965 Jan;89:28–40. doi: 10.1128/jb.89.1.28-40.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
  5. Faur Y. C., Weisburd M. H., Wilson M. E., May P. S. A new medium for the isolation of pathogenic Neisseria (NYC medium). I. Formulation and comparisons with standard media. Health Lab Sci. 1973 Apr;10(2):44–54. [PubMed] [Google Scholar]
  6. Hunter K. M., McVeigh I. Development of a chemically defined medium for growth of Neisseria gonorrhoeae. Antonie Van Leeuwenhoek. 1970;36(2):305–316. doi: 10.1007/BF02069032. [DOI] [PubMed] [Google Scholar]
  7. Kellogg D. S., Jr, Cohen I. R., Norins L. C., Schroeter A. L., Reising G. Neisseria gonorrhoeae. II. Colonial variation and pathogenicity during 35 months in vitro. J Bacteriol. 1968 Sep;96(3):596–605. doi: 10.1128/jb.96.3.596-605.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. La Scolea L. J., Jr, Young F. E. Development of a defined minimal medium for the growth of Neisseria gonorrhoeae. Appl Microbiol. 1974 Jul;28(1):70–76. doi: 10.1128/am.28.1.70-76.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Ley H. L., Jr, Mueller J. H. On the Isolation from Agar of an Inhibitor for Neisseria gonorrhoeae. J Bacteriol. 1946 Oct;52(4):453–460. doi: 10.1128/jb.52.4.453-460.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Martin J. E., Jr, Billings T. E., Hackney J. F., Thayer J. D. Primary isolation of N. gonorrhoeae with a new commercial medium. Public Health Rep. 1967 Apr;82(4):361–363. [PMC free article] [PubMed] [Google Scholar]
  11. Morse S. A., Bartenstein L. Factors affecting autolysis of Neisseria gonorrhoeae. Proc Soc Exp Biol Med. 1974 Apr;145(4):1418–1421. doi: 10.3181/00379727-145-38025. [DOI] [PubMed] [Google Scholar]
  12. Walstad D. L., Reitz R. C., Sparling P. F. Growth inhibition among strains of Neisseria gonorrhoeae due to production of inhibitory free fatty acids and lysophosphatidylethanolamine: absence of bacteriocins. Infect Immun. 1974 Sep;10(3):481–488. doi: 10.1128/iai.10.3.481-488.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES