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. 1977 Jan;5(1):86–90. doi: 10.1128/jcm.5.1.86-90.1977

Contained indirect viable-cell membrane immunofluorescence microassay for surface antigen analysis of cells infected with hazardous viruses.

M W Cloyd, D D Bigner
PMCID: PMC274537  PMID: 188865

Abstract

A microtechnique for an indirect viable-cell membrane immunofluorescence titration assay was developed using Friend murine leukemia virus (F-MuLV)-producing cells and monospecific rabbit antisera to F-MuLV structural antigens. The assay was sensitive and displayed little variation within or between assays. Since moderate-risk tumor viruses, such as recently discovered primate oncornaviruses or feline leukemia virus (FeLV), may be hazardous to laboratory personnel, the assay was adapted for containment of cells infected with such viruses. Cells producing gibbon ape lymphoma virus or FeLV were grown in class III containment cabinets and transferred in sealed flasks to a class II laminar-flow cabinet, where the assay was performed. This micromethod not only conserved reagents but also minimized the numbers of moderate-risk tumor virus-infected cells handled at one time. Centrifugation was contained using custom-made devices shown to form a gas-tight seal over microtiter plates. Interspecies reactivity of monospecific rabbit antisera against F-MuLV structural antigen gp71, but not against p12, was demonstrated for surface antigens on FeLV-producing cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Avrameas S. Immunoenzyme techniques: enzymes as markers for the localization of antigens and antibodies. Int Rev Cytol. 1970;27:349–385. doi: 10.1016/s0074-7696(08)61250-4. [DOI] [PubMed] [Google Scholar]
  2. Baldwin R. W., Barker C. R., Embleton M. J., Glaves D., Moore M., Pimm M. V. Demonstration of cell-surface antigens on chemically induced tumors. Ann N Y Acad Sci. 1971 Jun 21;177:268–278. doi: 10.1111/j.1749-6632.1971.tb35056.x. [DOI] [PubMed] [Google Scholar]
  3. Boyse E. A., Hubbard L., Stockert E., Lamm M. E. Improved complementation in the cytotoxic test. Transplantation. 1970 Nov;10(5):446–449. doi: 10.1097/00007890-197011000-00019. [DOI] [PubMed] [Google Scholar]
  4. COONS A. H., KAPLAN M. H. Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody. J Exp Med. 1950 Jan 1;91(1):1–13. doi: 10.1084/jem.91.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Grant J. P., Bigner D. D., Fischinger P. J., Bolognesi D. P. Expression of murine leukemia virus structural antigens on the surface of chemically induced murine sarcomas. Proc Natl Acad Sci U S A. 1974 Dec;71(12):5037–5041. doi: 10.1073/pnas.71.12.5037. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hunsmann G., Claviez M., Moennig V., Schwarz H., Schäfer W. Properties of mouse leukemia viruses. X. Occurrence of viral structural antigens on the cell surface as revealed by a cytotoxicity test. Virology. 1976 Jan;69(1):157–168. doi: 10.1016/0042-6822(76)90203-8. [DOI] [PubMed] [Google Scholar]
  7. Hunsmann G., Moennig V., Pister L., Seifert E., Schäfer W. Properties of mouse leukemia viruses. VIII. The major viral glycoprotein of Friend leukemia virus. Seroimmunological, interfering and hemagglutinating capacities. Virology. 1974 Dec;62(2):307–318. doi: 10.1016/0042-6822(74)90394-8. [DOI] [PubMed] [Google Scholar]
  8. Ikeda H., Pincus T., Yoshiki T., Strand M., August J. T., Boyse E. A., Mellors R. C. Biological expression of antigenic determinants of murine leukemia virus proteins gp69-71 and p30. J Virol. 1974 Nov;14(5):1274–1280. doi: 10.1128/jvi.14.5.1274-1280.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. KLEIN E., KLEIN G. ANTIGENIC PROPERTIES OF LYMPHOMAS INDUCED BY THE MOLONEY AGENT. J Natl Cancer Inst. 1964 Mar;32:547–568. [PubMed] [Google Scholar]
  10. Kawakami T. G., Huff S. D., Buckley P. M., Dungworth D. L., Synder S. P., Gilden R. V. C-type virus associated with gibbon lymphosarcoma. Nat New Biol. 1972 Feb 9;235(58):170–171. doi: 10.1038/newbio235170a0. [DOI] [PubMed] [Google Scholar]
  11. Klein E., Klein G., Nadkarni J. S., Nadkarni J. J., Wigzell H., Clifford P. Surgace IgM specificity on cells derived from a Burkitt's lymphoma. Lancet. 1967 Nov 18;2(7525):1068–1070. doi: 10.1016/s0140-6736(67)90340-6. [DOI] [PubMed] [Google Scholar]
  12. Levy N. L., Amos D. B., Solovieff G. V., dos Reis A. P. In vitro methods for assessment of antibody-mediated tumor immunity. Natl Cancer Inst Monogr. 1972 Dec;35:5–11. [PubMed] [Google Scholar]
  13. McCormick N. K., McCormick K. J., Trentin J. J. Microtechnique for indirect immunofluorescence. Appl Microbiol. 1973 Dec;26(6):1015–1015. doi: 10.1128/am.26.6.1015-1015.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pancake S. J., Mora P. T. Limitations and utility of a cytolytic assay for measuring simian virus 40-induced cell surface antigens. Cancer Res. 1976 Jan;36(1):88–94. [PubMed] [Google Scholar]
  15. Rickard C. G., Post J. E., Noronha F., Barr L. M. A transmissible virus-induced lymphocytic leukemia of the cat. J Natl Cancer Inst. 1969 Jun;42(6):987–1014. [PubMed] [Google Scholar]
  16. Schwarz H., Hunsmann G., Moenning V., Schäfer W. Properties of mouse leukemia viruses. XI. Immunoelectron microscopic studies on viral structural antigens on the cell surface. Virology. 1976 Jan;69(1):169–178. doi: 10.1016/0042-6822(76)90204-x. [DOI] [PubMed] [Google Scholar]
  17. Seifert E., Claviez M., Frank H., Hunsmann G., Schwarz H., Schäfer W. XII. Produktion grösserer Mengen von Friend-Virus durch eine permanente Zell-Suspensions-Kultur (Eveline-Suspensions-Zellen) Z Naturforsch C. 1975 Sep-Oct;30(5):698–700. [PubMed] [Google Scholar]
  18. Strand M., August J. T. Structural proteins of mammalian oncogenic RNA viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein. J Virol. 1974 Jan;13(1):171–180. doi: 10.1128/jvi.13.1.171-180.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Strand M., August J. T. Structural proteins of oncogenic ribonucleic acid viruses. Interspec II, a new interspecies antigen. J Biol Chem. 1973 Aug 25;248(16):5627–5633. [PubMed] [Google Scholar]
  20. Yoshiki T., Mellors R. C., Hardy W. D., Jr Common cell-surface antigen associated with murine and feline C-type RNA leukemia viruses. Proc Natl Acad Sci U S A. 1973 Jun;70(6):1878–1882. doi: 10.1073/pnas.70.6.1878. [DOI] [PMC free article] [PubMed] [Google Scholar]

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