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. 1977 Jan;5(1):100–105. doi: 10.1128/jcm.5.1.100-105.1977

Inhibition of immune hemolysis: serological assay for the heat-labile enterotoxin of Excherichia coli.

D J Evans Jr, D G Evans
PMCID: PMC274539  PMID: 319105

Abstract

Sheep erythrocytes sensitized by incubation with the heat-labile enterotoxin (LT) of Escherichia coli are hemolyzed in the presence of anti-LT antiserum and complement. The Microtiter (Cooke Laboratory Products) technique was used to titrate anti-LT antibody in serum by this immune hemolysis reaction. Immune hemolysis was inhibited by preexposure of the anti-LT antiserum to soluble LT before addition of the LT-sensitized sheep cells. E. coli mini-extract preparations were obtained by the polymyxin release technique and assayed for LT by the lysis inhibition test (LIT) and by the adrenal cell assay. All 75 adrenal cell-positive E. coli isolates were positive in the LIT assay. Eight of 318 adrenal cell-negative isolates tested were positive in the LIT assay, possibly indicating the presence of biologically inactive toxin.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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