Abstract
A total of 627 clinical isolates of Micrococcaceae were characterized accoridng to their ability to coagulate rabbit and pig plasma, produce thermostable nuclease, and anaerobically ferment glucose and mannitol. By using these characteristics, 416 of the isolates were classified as Staphylococcus aureus and 211 as non-S. aureus. All 416 strains produced a 3 to 4+ clot formation in heparinized pig plasma, whereas 415 isolates produced a similar reaction in citrated rabbit plasma. All of the S. aureus strains possessed a thermostable nuclease, whereas only four of the non-S. aureus isolates exhibited this characteristic. The results obtained using heparinized pig plasma were almost identical to those obtained with commercial rabbit plasma.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Barry A. L., Lachica R. V., Atchison F. W. Identification of Staphylococcus aureus by simultaneous use of tube coagulase and thermonuclease tests. Appl Microbiol. 1973 Mar;25(3):496–497. doi: 10.1128/am.25.3.496-497.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lachica R. V., Genigeorgis C., Hoeprich P. D. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl Microbiol. 1971 Apr;21(4):585–587. doi: 10.1128/am.21.4.585-587.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parisi J. T., Baldwin J. N., Sottile M. Pour-plate method for the detection of coagulase production by Staphylococcus aureus. Appl Microbiol. 1973 Apr;25(4):558–561. doi: 10.1128/am.25.4.558-561.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rayman M. K., Park C. E., Philpott J., Todd E. C. Reassessment of the coagulase and thermostable nuclease tests as means of identifying Staphylococcus aureus. Appl Microbiol. 1975 Apr;29(4):451–454. doi: 10.1128/am.29.4.451-454.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sperber W. H., Tatini S. R. Interpretation of the tube coagulase test for identification of Staphylococcus aureus. Appl Microbiol. 1975 Apr;29(4):502–505. doi: 10.1128/am.29.4.502-505.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TURNER F. J., SCHWARTZ B. S. The use of a lyophilized human plasma standardized for blood coagulation factors in the coagulase and fibrinolytic tests. J Lab Clin Med. 1958 Dec;52(6):888–894. [PubMed] [Google Scholar]
- Victor R., Lachica F., Weiss K. F., Deibel R. H. Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus. Appl Microbiol. 1969 Jul;18(1):126–127. doi: 10.1128/am.18.1.126-127.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]