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. 2009 Oct;11(10):1042–1053. doi: 10.1593/neo.09760

Figure 7.

Figure 7

Decorin induced apoptosis by targeting EGFR and AR. (A) PC3 cells were treated with decorin (2 µM) or AG1478 (2 µM, pretreated for 30 minutes) followed by EGF (100 ng/ml) for 48 hours. Caspase 3 activity was measured with the Caspase-Glo 3/7 assay. Values representing the mean ± SD (n = 3) with different letters are significantly different (P < .05). (B) LNCaP cells were treated with decorin (2 µM) for 48 hours in the presence or absence of pretreatment with AG1478 (2 µM for 30 minutes), bicalutamide (0.5 µM for 90 minutes), or a combination of both. Caspase 3 activity was measured with the Caspase-Glo 3/7 assay. Values representing the mean ± SD (n = 3) with different letters are significantly different (P < .05). (C) PC3 cells were treated with decorin or the EGFR inhibitor, AG1478 (2 µM, pretreated for 30 minutes), for 48 hours, then cleaved PARP was measured by Western blot assay. Data shown are representative of two experiments with similar results. (D) LNCaP cells were treated with decorin for 72 hours in the presence or absence of pretreatment with AG1478 (2 µM for 30 minutes), bicalutamide (0.5 µM for 90 minutes), or a combination of both. Cleaved PARP was identified by Western analysis. Data shown (C and D) are representative of two independent experiments.