Abstract
The enzyme-linked immunosorbent assay (ELISA) has proved to be a sensitive and specific quantitative procedure for the serodiagnosis of toxoplasmosis. Using the toxoplasma model, several parameters of the test were investigated. Day-to-day reproducibility was 90% within one twofold dilution and 98% specific when tested against batteries of sera from other diseases. Both the tube method and the microtitration method were used successfully. ELISA results are equivalent to those found in the indirect immunofluorescence test, yet the ELISA procedure is simpler and more rapid to perform.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bos H. J., Eijk A. A., Steerenberg P. A. Letter: Application of ELISA--enzyme linked immunosorbent assay--in the sero-diagnosis of amoebiasis. Trans R Soc Trop Med Hyg. 1975;69(4):440–440. doi: 10.1016/0035-9203(75)90202-3. [DOI] [PubMed] [Google Scholar]
- Bout D., Dugimont J. C., Farag H., Capron A. Le diagnostic immunoenzymologique des affections parasitaires. II - Immunoenzymologie quantitative sur lame. Lille Med. 1975 Jun-Jul;20(6):561–566. [PubMed] [Google Scholar]
- Carlsson H. E., Lindberg A. A., Hammarström S., Ljunggren A. Quantitation of Salmonella O-antibodies in human sera by enzyme-linked immunosorbent assay (ELISA). Int Arch Allergy Appl Immunol. 1975;48(4):485–494. doi: 10.1159/000231336. [DOI] [PubMed] [Google Scholar]
- Engvall E., Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry. 1971 Sep;8(9):871–874. doi: 10.1016/0019-2791(71)90454-x. [DOI] [PubMed] [Google Scholar]
- Miedema K., Boelhouwer J., Otten J. W. Determinations of proteins and hormones in serum by an immunoassay using antigen-enzyme conjugates. Clin Chim Acta. 1972 Aug;40(1):187–192. doi: 10.1016/0009-8981(72)90269-0. [DOI] [PubMed] [Google Scholar]
- Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
- Ruitenberg E. J., Ljungström I., Steerenberg P. A., Buys J. Application of immunofluorescence and immunoenzyme methods in the serodiagnosis of Trichinella spiralis infection. Ann N Y Acad Sci. 1975 Jun 30;254:296–303. doi: 10.1111/j.1749-6632.1975.tb29179.x. [DOI] [PubMed] [Google Scholar]
- Ruitenberg E. J., Steerenberg P. A., Brosi B. J., Buys J. Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays. Bull World Health Organ. 1974;51(1):108–109. [PMC free article] [PubMed] [Google Scholar]
- Voller A., Bidwell D. E., Bartlett A., Fleck D. G., Perkins M., Oladehin B. A microplate enzyme-immunoassay for toxoplasma antibody. J Clin Pathol. 1976 Feb;29(2):150–153. doi: 10.1136/jcp.29.2.150. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Voller A., Draper C., Bidwell D. E., Bartlett A. Microplate enzyme-linked immunosorbent assay for chagas' disease. Lancet. 1975 Feb 22;1(7904):426–428. doi: 10.1016/s0140-6736(75)91492-0. [DOI] [PubMed] [Google Scholar]
- Voller A., Huldt G., Thors C., Engvall E. New serological test for malaria antibodies. Br Med J. 1975 Mar 22;1(5959):659–661. doi: 10.1136/bmj.1.5959.659. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Weemen B. K., Schuurs A. H. Immunoassay using hapten--enzyme conjugates. FEBS Lett. 1972 Jul 15;24(1):77–81. doi: 10.1016/0014-5793(72)80830-5. [DOI] [PubMed] [Google Scholar]