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. 1977 Mar;5(3):278–284. doi: 10.1128/jcm.5.3.278-284.1977

Inactivation of classical and alternative pathway-activated bactericidal activity of human serum by sodium polyanetholsulfonate.

W H Traub, I Kleber
PMCID: PMC274582  PMID: 192756

Abstract

Sodium polyanetholsulfonate (SPS) at a final concentration of at least 250 microng/ml (0.025%) was required for inhibition of the bactericidal activity of 80% (vol/vol) of fresh human serum against "promptly serum-sensitive" strains of Serratia marcescens and control strain Escherichia coli C, i.e., for inhibition of the classical pathway of complement activation. In contrast, SPS at 125 microng/ml (0.0125%) was sufficient for neutralization of the bactericidal activity of 80% (vol/vol) fresh human serum against "delayed serum-sensitive" strains of S. marcescens known to activate the alternative pathway of human complement. Addition of up to 500 microng of SPS per ml to 80% (vol/vol) fresh human serum failed to neutralize transferrin-mediated, "late" bacteriostasis against control strain E. coli C, an effect that was demonstrable only after prolonged, i.e., overnight, incubation of the test strain. However, this late inhibitory effect against E. coli C was not observed in SPS-treated 20% (vol/vol) fresh human serum or in 10 or 20% (vol/vol) conventionally heat-inactivated human serum. Immunoelectrophoretic examination disclosed that SPS did not precipitate transferrin from either fresh or heat-inactivated human serum. Thus, SPS, at 250 microng/ml, was demonstrated to be sufficient for the inhibition of both classical and alternative complement pathway-activated bactericidal activity of 80% (vol/vol) human serum. However, SPS at a concentration of 500 microng/ml failed to antagonize one antimicrobial system of 80% (vol/vol) human serum, namely transferrin-mediated bacteriostasis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Des Prez R. M., Bryan C. S., Hawiger J., Colley D. G. Function of the classical and alternate pathways of human complement in serum treated with ethylene glycol tetraacetic acid and MgCl2-ethylene glycol tetraacetic acid. Infect Immun. 1975 Jun;11(6):1235–1243. doi: 10.1128/iai.11.6.1235-1243.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fine D. P. Activation of the classic and alternate complement pathways by endotoxin. J Immunol. 1974 Feb;112(2):763–769. [PubMed] [Google Scholar]
  3. Fine D. P., Marney S. R., Jr, Colley D. G., Sergent J. S., Des Prez R. M. C3 shunt activation in human serum chelated with EGTA. J Immunol. 1972 Oct;109(4):807–809. [PubMed] [Google Scholar]
  4. Goodkofsky I., Lepow I. H. Functional relationship of factor B in the properdin system to C3 proactivator of human serum. J Immunol. 1971 Oct;107(4):1200–1204. [PubMed] [Google Scholar]
  5. KLEIN P., LANGE A. Uber die Reaktivierung von Komplement nach Vergiftung durch hochmolekulare Anticoagulantien. Z Hyg Infektionskr. 1956;142(5):445–456. [PubMed] [Google Scholar]
  6. KLEIN P. Untersuchungen über den Angriffspunkt von gerinnungshemmenden Stoffen am Komplement. Z Hyg Infektionskr. 1956;142(5):457–475. [PubMed] [Google Scholar]
  7. Loos M., Volanakis J. E., Stroud R. M. Mode of interaction of different polyanions with the first (C1, C1), the second (C2) and the fourth (C4) component of complement--II. Effect of polyanions on the binding of C2 to EAC4b. Immunochemistry. 1976 Mar;13(3):257–261. doi: 10.1016/0019-2791(76)90224-x. [DOI] [PubMed] [Google Scholar]
  8. PONTIERI G. M., COTRUFO M., CICCIMARRA F., TOLONE G. ATTEMPTS TO ISOLATE C'3 ACTIVITY FROM PIG SERUM. Experientia. 1965 Feb 15;21:75–76. doi: 10.1007/BF02144749. [DOI] [PubMed] [Google Scholar]
  9. Raepple E., Hill H. U., Loos M. Mode of interaction of different polyanions with the first (C1, C1), the second (C2) and the fourth (C4) component of complement--I. Effect on fluid phase C1 and on C1 bound to EA or to EAC4. Immunochemistry. 1976 Mar;13(3):251–255. doi: 10.1016/0019-2791(76)90223-8. [DOI] [PubMed] [Google Scholar]
  10. Root R. K., Ellman L., Frank M. M. Bactericidal and opsonic properties of C4-deficient guinea pig serum. J Immunol. 1972 Sep;109(3):477–486. [PubMed] [Google Scholar]
  11. Sandberg A. L., Osler A. G. Dual pathways of complement interaction with guinea pig immunoglobulins. J Immunol. 1971 Nov;107(5):1268–1273. [PubMed] [Google Scholar]
  12. Traub W. H. Assay of the antibiotic activity of serum. Appl Microbiol. 1969 Jul;18(1):51–56. doi: 10.1128/am.18.1.51-56.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Traub W. H., Kleber I. Selective activation of classical and alternative pathways of human complement by "promptly serum-sensitive" and "delayed serum-sensitive" strains of Serratia marcescens. Infect Immun. 1976 May;13(5):1343–1346. doi: 10.1128/iai.13.5.1343-1346.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Traub W. H., Kleber I. Studies on the additive effect of polymyxin B and the bactericidal activity of human serum against Serratia marcescens. Chemotherapy. 1975;21(3-4):189–204. doi: 10.1159/000221860. [DOI] [PubMed] [Google Scholar]
  15. Traub W. H., Lowrance B. L. Anticomplementary, anticoagulatory, and serum-protein precipitating activity of sodium polyanetholsulfonate. Appl Microbiol. 1970 Sep;20(3):465–468. doi: 10.1128/am.20.3.465-468.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Weinberg E. D. Iron and susceptibility to infectious disease. Science. 1974 May 31;184(4140):952–956. doi: 10.1126/science.184.4140.952. [DOI] [PubMed] [Google Scholar]
  17. Wilkins T. D., West S. E. Medium-dependent inhibition of Peptostreptococcus anaerobius by sodium polyanetholsulfonate in blood culture media. J Clin Microbiol. 1976 Apr;3(4):393–396. doi: 10.1128/jcm.3.4.393-396.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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