Abstract
Two simplified media for isolating Neisseria gonorrhoeae are described. They differ from the modified Thayer-Martin medium in the enrichment and antibiotics used for preparation. The enrichment for one, enrichment 4 medium, contains only four ingredients, and amphotericin B is used instead of nystatin. This medium is comparable to the modified Thayer-Martin medium for isolating N.gonorrhoeae. It is convenient to prepare and is only one-third the cost of Thayer Martin medium. It is a suitable alternative to the modified Thayer-Martin. The enrichment 5 medium is a hemoglobin-free version of enrichment 4 medium. It is somewhat more selective for contaminants but is also more inhibitory for N. gonorrhoeae.
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- Faur Y. C., Weisburd M. H., Wilson M. E. A new medium for the isolation of pathogenic Neisseria (NYC medium). II. Effect of amphotericin B and trimethoprim lactate on selectivity. Health Lab Sci. 1973 Apr;10(2):55–60. [PubMed] [Google Scholar]
- Jacobs N. F., Jr, Kraus S. J. Comparison of hemoglobin-free culture media and thayer-martin medium for the primary isolation of Neisseria gonorrhoeae. J Clin Microbiol. 1975 May;1(5):401–404. doi: 10.1128/jcm.1.5.401-404.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Martin J. E., Armstrong J. H., Smith P. B. New system for cultivation of Neisseria gonorrhoeae. Appl Microbiol. 1974 Apr;27(4):802–805. doi: 10.1128/am.27.4.802-805.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- THAYER J. D., MARTIN J. E., Jr A SELECTIVE MEDIUM FOR THE CULTIVATION OF N. GONORRHOEAE AND N. MENINGITIDIS. Public Health Rep. 1964 Jan;79:49–57. [PMC free article] [PubMed] [Google Scholar]
- White L. A., Kellogg D. S., Jr An improved fermentation medium for Neisseria gonorrhoeae and other Neisseria. Health Lab Sci. 1965 Oct;2(4):238–241. [PubMed] [Google Scholar]