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. 1977 Apr;5(4):401–404. doi: 10.1128/jcm.5.4.401-404.1977

Cellular immunity in chronic Chagas' disease.

O M Montufar, C C Musatti, E Mendes, N F Mendes
PMCID: PMC274614  PMID: 404317

Abstract

The cellular immune response was assessed in 20 patients with chronic Chagas' disease (American trypanosomiasis). Thymus-derived lymphocyte function was determined in vivo by cutaneous reactivity to several antigens including a soluble preparation derived from Trypanosoma cruzi and sensitization to 2,4-dinitrochlorobenzene. The in vitro T-cell reactivity was investigated by the proliferative response to phytohemagglutinin and to T. cruzi antigen and by inhibition of leukocyte migration with the specific antigen. In addition, the proportion and absolute numbers of peripheral blood T and B-lymphocytes were determined by rosette formation. This research indicates that the general and specific cellular immune response, evaluated by the tests herein mentioned, is well preserved in patients, with Chagas' disease. We conclude that chronic Chagas' disease is not associated with deficiency in cellular immunity, nor does it lead to it. Conceivably, the active participation of delayed hypersensitivity may play an important role in the expression of the human chagasic lesions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brener Z., Chiari E. The effects of some immunosuppressive agents in experimental chronic Chagas's disease. Trans R Soc Trop Med Hyg. 1971;65(5):629–636. doi: 10.1016/0035-9203(71)90047-2. [DOI] [PubMed] [Google Scholar]
  2. Galvão M. M., Sabbaga E., Peixinho Z. F., Musatti C. C., Mendes N. F. Immunosuppressive effect of intra-lymphatic irradiation. Allergol Immunopathol (Madr) 1975 Sep-Oct;3(5):299–308. [PubMed] [Google Scholar]
  3. González Cappa S. M., Schmuñis G. A., Traversa O. C., Yanovsky J. F., Parodi A. S. Complement-fixation tests, skin tests, and experimental immunization with antigens of Trypanosoma cruzi prepared under pressure. Am J Trop Med Hyg. 1968 Sep;17(5):709–715. doi: 10.4269/ajtmh.1968.17.709. [DOI] [PubMed] [Google Scholar]
  4. Krettli A. U., Brener Z. Protective effects of specific antibodies in Trypanosoma cruzi infections. J Immunol. 1976 Mar;116(3):755–760. [PubMed] [Google Scholar]
  5. Kuhn R. E., Durum S. K. The onset of immune protection in acute experimental Chagas' disease in C3H(HE) mice. Int J Parasitol. 1975 Apr;5(2):241–244. doi: 10.1016/0020-7519(75)90113-7. [DOI] [PubMed] [Google Scholar]
  6. Lelchuk R., Patrucco A., Manni J. A. Studies of cellular immunity in Chagas disease: effect of glutaraldehyde-treated specific antigen on inhibition of leukocyte migration. J Immunol. 1974 Apr;112(4):1578–1581. [PubMed] [Google Scholar]
  7. MUNIZ J. [Immunity in Chagas' disease (trypanosomiasis americana)]. Mem Inst Oswaldo Cruz. 1962 Mar;60:103–147. doi: 10.1590/s0074-02761962000100006. [DOI] [PubMed] [Google Scholar]
  8. Mendes E., Raphael A. Impaired delayed hypersensitivity in patients with South American blastomycosis. J Allergy. 1971 Jan;47(1):17–22. doi: 10.1016/s0091-6749(71)80313-5. [DOI] [PubMed] [Google Scholar]
  9. Mendes N. F., Miki S. S., Peixinho Z. F. Combined detection of human T and B lymphocytes by rosette formation with sheep erythrocytes and zymosan-C3 complexes. J Immunol. 1974 Aug;113(2):531–536. [PubMed] [Google Scholar]
  10. Mendes N. F., Musatti C. C., Leão R. C., Mendes E., Naspitz C. K. Lymphocyte cultures and skin allograft survival in patients with South American blastomycosis. J Allergy Clin Immunol. 1971 Jul;48(1):40–45. doi: 10.1016/0091-6749(71)90054-6. [DOI] [PubMed] [Google Scholar]
  11. Mendes N. F., Musatti C. C., Tolnai M. E. T and B lymphocyte membrane markers in cells from patients with leukemia and lymphoma. Int Arch Allergy Appl Immunol. 1974;46(5):695–706. doi: 10.1159/000231170. [DOI] [PubMed] [Google Scholar]
  12. Mendes N. F., Tolnai M. E., Silveira N. P., Gilbertsen R. B., Metzgar R. S. Technical aspects of the rosette tests used to detect human complement receptor (B) and sheep erythrocyte-binding (T) lymphocytes. J Immunol. 1973 Sep;111(3):860–867. [PubMed] [Google Scholar]
  13. Musatti C. C., Rezkallah M. T., Mendes E., Mendes N. F. In vivo and in vitro evaluation of cell-mediated immunity in patients with paracoccidiodomycosis. Cell Immunol. 1976 Jun 15;24(2):365–378. doi: 10.1016/0008-8749(76)90220-3. [DOI] [PubMed] [Google Scholar]
  14. Roberson E. L., Hanson W. L. Correspondence: Transfer of immunity to T. cruzi. Trans R Soc Trop Med Hyg. 1974;68(4):338–338. doi: 10.1016/0035-9203(74)90044-3. [DOI] [PubMed] [Google Scholar]
  15. Roberson E. L., Hanson W. L. Trypanosoma cruzi: effects of anti-thymocyte serum in mice and neonatal thymectomy in rats. Exp Parasitol. 1973 Oct;34(2):168–180. doi: 10.1016/0014-4894(73)90076-3. [DOI] [PubMed] [Google Scholar]
  16. Santos-Buch C. A., Teixeira A. R. The immunology of experimental Chagas' disease. 3. Rejection of allogeneic heart cells in vitro. J Exp Med. 1974 Jul 1;140(1):38–53. doi: 10.1084/jem.140.1.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schmunis G. A., Cappa S. M., Traversa O. C., Janovsky J. F. The effect of immuno-depression due to neonatal thymectomy on infections with Trypanosoma cruzi in mice. Trans R Soc Trop Med Hyg. 1971;65(1):89–94. doi: 10.1016/0035-9203(71)90190-8. [DOI] [PubMed] [Google Scholar]
  18. Teixeira A. R., Santos-Buch C. A. The immunology of experimental Chagas' disease. I. Preparation of Trypanosoma cruzi antigens and humoral antibody response to there antigens. J Immunol. 1974 Sep;113(3):859–869. [PubMed] [Google Scholar]
  19. Tschudi E. I., Anziano D. F., Dalmasso A. P. Lymphocyte transformation in Chagas disease. Infect Immun. 1972 Dec;6(6):905–908. doi: 10.1128/iai.6.6.905-908.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yanovsky J. F., Albado E. Humoral and cellular responses to Trypanosoma cruzi infection. J Immunol. 1972 Nov;109(5):1159–1161. [PubMed] [Google Scholar]
  21. Zeledón R., Ponce C. Letter: A skin test for the diagnosis of Chagas's disease. Trans R Soc Trop Med Hyg. 1974;68(5):414–415. doi: 10.1016/0035-9203(74)90164-3. [DOI] [PubMed] [Google Scholar]

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