Abstract
Methods are described for the rapid detection of beta-D-glucosidase and phosphatase in mycoplasma cultures using fluorogenic 4-methylumbelliferone substrates. These methods were applied to a selection of mycoplasma cultures and were compared with the conventionally used tests for these enzymes. Results were similar by both methods, but the fluorogenic tests could be read after 1 h, whereas the conventional tests took several days.
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- Bradbury J. M., Oriel C. A., Jordan F. T. Simple method for immunofluorescent identification of mycoplasma colonies. J Clin Microbiol. 1976 Apr;3(4):449–452. doi: 10.1128/jcm.3.4.449-452.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Erno H., Stipkovits L. Bovine mycoplasmas: cultural and biochemical studies. II. Acta Vet Scand. 1973;14(3):450–463. doi: 10.1186/BF03547432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fabricant J., Freundt E. A. Importance of extension and standardization of laboratory tests for the identification and classification of mycoplasma. Ann N Y Acad Sci. 1967 Jul 28;143(1):50–58. doi: 10.1111/j.1749-6632.1967.tb27643.x. [DOI] [PubMed] [Google Scholar]
- Jacks T. J., Kircher H. W. Fluorometric assay for the hydrolytic activity of lipase using fatty acyl esters of 4-methylumbelliferone. Anal Biochem. 1967 Nov;21(2):279–285. doi: 10.1016/0003-2697(67)90190-x. [DOI] [PubMed] [Google Scholar]
- Maddocks J. L., Greenan M. J. A rapid method for identifying bacterial enzymes. J Clin Pathol. 1975 Aug;28(8):686–687. doi: 10.1136/jcp.28.8.686. [DOI] [PMC free article] [PubMed] [Google Scholar]