Abstract
The effectiveness of counterimmunoelectrophoresis (CIEP) for detecting human precipitating antibodies to mcyoplasma antigen was compared with the conventional complement fixation (CF) method in a double-blind experiment. Fifty-one sera from patients suspected of having acute mycoplasma infection were tested by both techniques. Dense precipitin lines to mycoplasma antigen developed in 28 sera with CIEP. Twenty-six of 28 had elevated CF titers to this antigen. No precipitin bands were observed in sera with low antibody titers to mycoplasma. These findings indicate that the CIEP test is a specific method for reliably detecting elevated serum CF antibody levels in patients with acute or recent mycoplasma infection.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Fortunato J., Goldschmidt B., Menonna J., Dowling P., Cook S. Rapid detection of antibodies to cytomegalovirus by counterimmunoelectrophoresis. J Infect Dis. 1977 Mar;135(3):432–437. doi: 10.1093/infdis/135.3.432. [DOI] [PubMed] [Google Scholar]
- Gocke D. J., Howe C. Rapid detection of Australia antigen by counterimmunoelectrophoresis. J Immunol. 1970 Apr;104(4):1031–1034. [PubMed] [Google Scholar]
- Goldschmidt B. L., Menonna J. P., Dowling P. C., Cook S. D. Rapid detection of mycoplasma antibody. J Immunol. 1976 Sep;117(3):1054–1055. [PubMed] [Google Scholar]
- Jeansson S. Differentiation between herpes simplex virus type 1 and type 2 strains by immunoelectroosmophoresis. Appl Microbiol. 1972 Jul;24(1):96–100. doi: 10.1128/am.24.1.96-100.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Murray H. W., Masur H., Senterfit L. B., Roberts R. B. The protean manifestations of Mycoplasma pneumoniae infection in adults. Am J Med. 1975 Feb;58(2):229–242. doi: 10.1016/0002-9343(75)90574-4. [DOI] [PubMed] [Google Scholar]
- Rytel M. W. Rapid diagnostic methods in infectious diseases. Adv Intern Med. 1975;20:37–60. [PubMed] [Google Scholar]