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. 1977 Jun;5(6):629–634. doi: 10.1128/jcm.5.6.629-634.1977

Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus.

H Schmitz, H W Doerr, D Kampa, A Vogt
PMCID: PMC274669  PMID: 195977

Abstract

A sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin M antibodies to cytomegalovirus is described. The results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. Horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. Nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei of cytomegalovirus-infected cells.

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Selected References

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