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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1977 Jul;6(1):4–9. doi: 10.1128/jcm.6.1.4-9.1977

Survival of chlamydiae after cooling to -196 degrees C.

M J Prentice, J Farrant
PMCID: PMC274687  PMID: 886008

Abstract

Factors influencing the survival of chlamydiae after freezing were reexamined. From the data presented, it is suggested that preservation of laboratory-grown chlamydiae is best achieved through the use of sucrose as the cryoprotective agent, in the presence of 10% serum. Dimethyl sulfoxide and glycerol are more toxic. The period of exposure to sucrose before freezing must be kept as short as possible and be at 4 degrees C rather than at room temperature. The rate of cooling during freezing in sucrose is not important; however, cooling at a rate slower than 1 degrees C/min should be avoided. Since chlamydial survival is increased by rapid thawing, the volume of the sample should be kept to a minimum. Thawed suspensions should be inoculated onto cell monolayers without delay. The application of these methods may increase the proportion of stored clinical specimens in which chlamydiae can be found.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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