Abstract
Six phages isolated from lysogenic cultures of Staphylococcus epidermidis were used to type cultures in our collection classified previously as "untypable" with our original typing set of 13 phages, new clinical isolates from Missouri, and clinical isolates from Connecticut, Georgia, Pennsylvania, and Virginia. Typability with the new set of 6 phages and with our 13 original phages was compared. For 203 cultures, 81 (39.9%) cultures were typable with the set of 6 phages and 73 (36.0%) cultures were typable with the set of 13 phages. When the two typing sets were combined, the percentage of typability for 425 cultures increased to 49.9%. compared with 35.7% with the original set of 13 phages. For 109 cultures from outstate, the percentage of typability was 56.9%, whereas that for 316 cultures from Missouri was 47.5%. When only cultures in biotype 1 were considered, the percentage of typability for 268 cultures with the combined set increased to 58.2%, compared with 39.5% with the original set of 13 phages. Analysis of individual reactions for all 425 cultures typed in our laboratory indicated that 11 of the 19 phages in the combined set accounted for the majority of the reactions, and a provisional set containing these 11 phages is proposed.
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Selected References
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- Baird-Parker A. C. The basis for the present classification of staphylococci and micrococci. Ann N Y Acad Sci. 1974 Jul 31;236(0):7–14. doi: 10.1111/j.1749-6632.1974.tb41478.x. [DOI] [PubMed] [Google Scholar]
- Blouse L., Kolonel L. N., Watkins C. A., Atherton J. M. Efficacy of phage typing epidemiologically related Staphylococcus epidermidis strains. J Clin Microbiol. 1975 Oct;2(4):318–321. doi: 10.1128/jcm.2.4.318-321.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dean B. A., Williams R. E., Hall F., Corse J. Phage typing of coagulase-negative staphylococci and micrococci. J Hyg (Lond) 1973 Jun;71(2):261–270. doi: 10.1017/s0022172400022737. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Males B. M., Rogers W. A., Jr, Parisi J. T. Virulence factors of biotypes of Staphylococcus epidermidis from clinical sources. J Clin Microbiol. 1975 Mar;1(3):256–261. doi: 10.1128/jcm.1.3.256-261.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Minshew B. H., Rosenblum E. D. Transduction of tetracycline resistance in Staphylococcus epidermidis. Antimicrob Agents Chemother. 1972 Jun;1(6):508–511. doi: 10.1128/aac.1.6.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parisi J. T., Talbot H. W., Jr Improved rapid plate method for the isolation of bacteriophages from lysogenic bacteria. Appl Microbiol. 1974 Sep;28(3):503–504. doi: 10.1128/am.28.3.503-504.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pulverer G., Pillich J., Klein A. New bacteriophages of Staphylococcus epidermidis. J Infect Dis. 1975 Nov;132(5):524–531. doi: 10.1093/infdis/132.5.524. [DOI] [PubMed] [Google Scholar]
- Schmidt W. C., Jeffries C. D. Bacteriophage typing of gram-negative rod-shaped bacteria. CRC Crit Rev Clin Lab Sci. 1975 Oct;6(3):201–246. doi: 10.3109/10408367509151571. [DOI] [PubMed] [Google Scholar]
- Speller D. C., Mitchell R. G. Coagulase-negative staphylococci causing endocarditis after cardiac surgery. J Clin Pathol. 1973 Jul;26(7):517–522. doi: 10.1136/jcp.26.7.517. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Talbot H. W., Jr, Parisi J. T. Phage typing of Staphylococcus epidermidis. J Clin Microbiol. 1976 May;3(5):519–523. doi: 10.1128/jcm.3.5.519-523.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Verhoef J., Winkler K. C., van Boven C. P. Characters of phages from coagulase-negative staphylococci. J Med Microbiol. 1971 Nov;4(4):413–424. doi: 10.1099/00222615-4-4-413. [DOI] [PubMed] [Google Scholar]