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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1977 Jul;6(1):19–22. doi: 10.1128/jcm.6.1.19-22.1977

Maintenance of viability and comparison of identification methods for influenza and other respiratory viruses of humans.

B D Baxter, R B Couch, S B Greenberg, J A Kasel
PMCID: PMC274690  PMID: 195979

Abstract

A comparison of Hanks balanced salt solution, veal infusion broth (VIB), and charcoal viral transport medium for maintaining viability of type A influenza virus indicated approximately equal survival of virus on all three media at -70 and 4 degrees C, whereas at 25 degrees C virus survived best in VIB. VIB supplemented with bovine serum albumin was used as transport medium in a community-wide surveillance of febrile respiratory disease for influenza viruses. Unfrozen throat swab specimens were placed in VIB and stored at 4 degrees C for up to 5 days without effect on isolation frequencies of either type A or type B influenza virus or type 1 or type 3 parainfluenza virus. Comparison of indirect immunofluorescence with hemadsorption for detection of type A influenza virus in rhesus monkey kidney cultures revealed a requirement for at least five fluorescing cells to eliminate false positive indirect immunofluorescence tests and at least 3 days of incubation to eliminate false negative tests when compared with hemadsorption at later times. Detection frequencies for the two methods after 2 and 3 days of incubation were not significantly different.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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