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. 1977 Jul;6(1):50–54. doi: 10.1128/jcm.6.1.50-54.1977

Preparation and evaluation of a noninfectious monkey pox virus vaccine.

R G Olsen, J R Blakeslee, L Mathes, J H Nakano
PMCID: PMC274696  PMID: 195980

Abstract

Monkey pox virus was mechanically disrupted by low temperature and high pressure into soluble and insoluble fractions. Soluble fractions elicited virus-neutralizing antibodies (1:20 to 1:160) in rabbits, whereas the insoluble (in saline) fractions did not (less than 1:5). No infectious virus was detected after the disruption procedure. Rhesus monkeys immunized with the soluble fraction elicited virus-neutralizing (1:1,200), complement-fixing (1:16), and hemagglutinating-inhibiting (1:80 to 1:160) antibody titers and were completely protected against monkey pox virus-induced disease. This model of monkey pox virus subunit vaccine preparation may prove to be useful in developing an efficacious noninfectious vaccinia vaccine for use in high-risk individuals.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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