Figure 8.

The fate of day 8 CD8⁺ peritoneal exudate lymphocytes (PEL) in vivo. Day 8 BALB/c anti-EL4 PEL were stained with CFSE, analysed for CD8⁺ CFSE⁺ cells, and then reinjected intraperitoneally (i.p.) into remaining mice. Parallel experiments were conducted with immunized transgenic 2C and C57BL/6 anti-LF mice. PEL from the 2C mice were extracted 8 or 11 days after immunization and reinjected i.p. into C57BL/6 mice of the same day, i.e. day 8 PEL were injected into day 8 mice. PEL were removed from the latter mice at different times and the CD8⁺/CFSE⁺ or 2C CD8⁺ population was analysed by fluorescence-activated cell sorting. Each experiment was performed twice.