Abstract
Diffuse fluorescence in an indirect fluorescent-antibody test for antibody to Aspergillus fumigatus was eliminated by using germlings, rather than hyphae, as antigen. The modified method yielded diagnostic titers larger than or equal to 1:32 in 24 of 25 known cases, was highly specific, and showed good correlation with clinical status and results of immunodiffusion. This technique, which uses the germling antigen, inactivation of test serum at 59 +/- 1 degrees C, and anti-immunoglobulin G conjugate, is a sensitive, specific, and reliable quantitative indicator of aspergillosis and is useful in following the course of the disease.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- AL-DOORY Y., GORDON M. A. APPLICATION OF FLUORESCENT-ANTIBODY PROCEDURES TO THE STUDY OF PATHOGENIC DEMATIACEOUS FUNGI. J Bacteriol. 1963 Aug;86:332–338. doi: 10.1128/jb.86.2.332-338.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coleman R. M., Kaufman L. Use of the immunodiffusion test in the serodiagnosis of aspergillosis. Appl Microbiol. 1972 Feb;23(2):301–308. doi: 10.1128/am.23.2.301-308.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Drouhet E. Aspects actuels des mycoses profondes. Nouvelles acquisitions dans le diagnostic mycologique et immunologique des infections mycosiques. Arch Roum Pathol Exp Microbiol. 1971 Mar;30(1):65–77. [PubMed] [Google Scholar]
- Gaafar H. A., D'Arcangelis D. C. Fluorescent antibody test for the serological diagnosis of gonorrhea. J Clin Microbiol. 1976 Apr;3(4):438–442. doi: 10.1128/jcm.3.4.438-442.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gordon M. A., Almy R. E., Greene C. H., Fenton J. W., 2nd Diagnostic mycoserology by immunoelectroosmophoresis: a general, rapid, and sensitive microtechnic. Am J Clin Pathol. 1971 Oct;56(4):471–474. doi: 10.1093/ajcp/56.4.471. [DOI] [PubMed] [Google Scholar]
- Parker J. D., Sarosi G. A., Doto I. L., Tosh F. E. Pulmonary aspergillosis in sanatoriums in the south central United States. A national communicable disease center cooperative mycoses study. Am Rev Respir Dis. 1970 Apr;101(4):551–557. doi: 10.1164/arrd.1970.101.4.551. [DOI] [PubMed] [Google Scholar]
- Schaefer J. C., Yu B., Armstrong D. An aspergillus immunodiffusion test in the early diagnosis of aspergillosis in adult leukemia patients. Am Rev Respir Dis. 1976 Mar;113(3):325–329. doi: 10.1164/arrd.1976.113.3.325. [DOI] [PubMed] [Google Scholar]
- The current status of serologic, immunologic and skin tests in the diagnosis of pulmonary mycoses. Report of the Committee on Fungus Diseases and Subcommittee on Criteria for Clinical Diagnosis--American College of Chest Physicians. Chest. 1973 Feb;63(2):259–270. [PubMed] [Google Scholar]
- Walter J. E., Jones R. D. Serologic tests in diagnosis of aspergillosis. Dis Chest. 1968 Jun;53(6):729–735. doi: 10.1378/chest.53.6.729. [DOI] [PubMed] [Google Scholar]
- Warnock D. W., Eldred G. F. Immunoglobulin classes of antibodies to Aspergillus fumigatus in patients with pulmonary aspergillosis. Sabouraudia. 1975 Jul;13(2):204–208. [PubMed] [Google Scholar]
- Warnock D. W. Indirect immunofluorescence test for the detection of Aspergillus fumigatus antibodies. J Clin Pathol. 1974 Nov;27(11):911–912. doi: 10.1136/jcp.27.11.911. [DOI] [PMC free article] [PubMed] [Google Scholar]