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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1977 Sep;6(3):244–248. doi: 10.1128/jcm.6.3.244-248.1977

Rapid method for auxotyping multiple strains of Neisseria gonorrhoeae.

H B Short, V B Ploscowe, J A Weiss, F E Young
PMCID: PMC274747  PMID: 409729

Abstract

A rapid method for auxotyping strains was developed that uses microtiter plates. This miniplate technique enables rapid identification of major auxotypes present in clinical strains. Additional growth requirements can be identified by adding individual amino acid supplements to complete gonococcal genetic medium. Analysis of 8 clones from 40 patients revealed that 10 had more than 1 auxotype. Deoxyribonucleic acid-mediated transformation can be used to establish whether the strains with apparently more than one auxotype are defective in the same locus in each of the involved biosynthetic pathways. Selection of more than one clone is required in precise epidemiological studies.

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Selected References

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  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  3. Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
  5. Hipp S. S., Lawton W. D., Chen N. C., Gaafar H. A. Inhibition of Neisseria gonorrhoeae by a factor produced by Candida albicans. Appl Microbiol. 1974 Jan;27(1):192–196. doi: 10.1128/am.27.1.192-196.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JUENKER A. P. Infections with multiple types of Salmonellae. Am J Clin Pathol. 1957 Jun;27(6):646–651. doi: 10.1093/ajcp/27.6.646. [DOI] [PubMed] [Google Scholar]
  7. Janik A., Juni E., Heym G. A. Genetic Transformation as a tool for detection of Neisseria gonorrhoeae. J Clin Microbiol. 1976 Jul;4(1):71–81. doi: 10.1128/jcm.4.1.71-81.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KELLOGG D. S., Jr, PEACOCK W. L., Jr, DEACON W. E., BROWN L., PIRKLE D. I. NEISSERIA GONORRHOEAE. I. VIRULENCE GENETICALLY LINKED TO CLONAL VARIATION. J Bacteriol. 1963 Jun;85:1274–1279. doi: 10.1128/jb.85.6.1274-1279.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Knapp J. S., Holmes K. K. Disseminated gonococcal infections caused by Neisseria gonorrhoeae with unique nutritional requirements. J Infect Dis. 1975 Aug;132(2):204–208. doi: 10.1093/infdis/132.2.204. [DOI] [PubMed] [Google Scholar]
  10. La Scolea L. J., Jr, Young F. E. Development of a defined minimal medium for the growth of Neisseria gonorrhoeae. Appl Microbiol. 1974 Jul;28(1):70–76. doi: 10.1128/am.28.1.70-76.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Morse S. A., Vaughan P., Johnson D., Iglewski B. H. Inhibition of Neisseria gonorrhoeae by a bacteriocin from Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1976 Aug;10(2):354–362. doi: 10.1128/aac.10.2.354. [DOI] [PMC free article] [PubMed] [Google Scholar]

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