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. 1977 Oct;6(4):328–331. doi: 10.1128/jcm.6.4.328-331.1977

Cultivation of Chlamydia trachomatis in cycloheximide-treated mccoy cells.

K T Ripa, P A Mårdh
PMCID: PMC274768  PMID: 562356

Abstract

An isolation technique for Chlamydia trachomatis using McCoy cells is described. In contrast to earlier techniques employing such cells, no pretreatment of the cells was used. The glutarimide antibiotic cycloheximide was added to the culture medium used for incubating the cells after infection. Cycloheximide was used at concentrations that depressed, but did not completely inhibit, the metabolism of the eucaryotic host cells. In studies on different immunotypes of C. trachomatis cultured in the yolk sac of embryonated hen eggs, the cycloheximide technique was compared with a method using pretreatment of cells with 5-iodo-2-deoxyuridine. The cycloheximide method gave greater numbers of inclusion-forming units per cover slip for all the immunotypes of trachoma-inclusion conjunctivitis agents tested, i.e., A through I. In a study of 194 cervical and urethral specimens from women, cycloheximide treatment of McCoy cells was found to be more efficient than 5-iodo-2-deoxyuridine treatment for the isolation of C. trachomatis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alexander J. J. Separation of protein synthesis in meningopneumonitisgent from that in L cells by differential susceptibility to cycloheximide. J Bacteriol. 1968 Feb;95(2):327–332. doi: 10.1128/jb.95.2.327-332.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Darougar S., Cubitt S., Jones B. R. Effect of high-speed centrifugation on the sensitivity of irradiated McCoy cell culture for the isolation of Chlamydia. Br J Vener Dis. 1974 Aug;50(4):308–312. doi: 10.1136/sti.50.4.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ennis H. L. Synthesis of ribonucleic acid in L cells during inhibition of protein synthesis by cycloheximide. Mol Pharmacol. 1966 Nov;2(6):543–557. [PubMed] [Google Scholar]
  4. GORDON F. B., MAGRUDER G. B., QUAN A. L., ARM H. G. Cell cultures for detection of trachoma virus from experimental simian infections. Proc Soc Exp Biol Med. 1963 Jan;112:236–242. doi: 10.3181/00379727-112-28004. [DOI] [PubMed] [Google Scholar]
  5. GORDON F. B., QUAN A. L. ISOLATION OF THE TRACHOMA AGENT IN CELL CULTURE. Proc Soc Exp Biol Med. 1965 Feb;118:354–359. doi: 10.3181/00379727-118-29841. [DOI] [PubMed] [Google Scholar]
  6. Gordon F. B., Harper I. A., Quan A. L., Treharne J. D., Dwyer R. S., Garland J. A. Detection of Chlamydia (Bedsonia) in certain infections of man. I. Laboratory procedures: comparison of yolk sac and cell culture for detection and isolation. J Infect Dis. 1969 Oct;120(4):451–462. doi: 10.1093/infdis/120.4.451. [DOI] [PubMed] [Google Scholar]
  7. Hatch T. P. Competition between Chlamydia psittaci and L cells for host isoleucine pools: a limiting factor in chlamydial multiplication. Infect Immun. 1975 Jul;12(1):211–220. doi: 10.1128/iai.12.1.211-220.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hobson D., Johnson F. W., Rees E., Tait I. A. Simplified method for diagnosis of genital and ocular infections with Chlamydia. Lancet. 1974 Sep 7;2(7880):555–556. doi: 10.1016/s0140-6736(74)91879-0. [DOI] [PubMed] [Google Scholar]
  9. Kuo C. C., Wang S. P., Grayston J. T. Effect of polycations, polyanions and neuraminidase on the infectivity of trachoma-inclusin conjunctivitis and lymphogranuloma venereum organisms HeLa cells: sialic acid residues as possible receptors for trachoma-inclusion conjunction. Infect Immun. 1973 Jul;8(1):74–79. doi: 10.1128/iai.8.1.74-79.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kuo C., Wang S., Wentworth B. B., Grayston J. T. Primary isolation of TRIC organisms in HeLa 229 cells treated with DEAE-dextran. J Infect Dis. 1972 Jun;125(6):665–668. doi: 10.1093/infdis/125.6.665. [DOI] [PubMed] [Google Scholar]
  11. Richmond S. J. Letter: Growth of Chlamydia trachomatis in cell culture. Lancet. 1976 Apr 17;1(7964):865–865. doi: 10.1016/s0140-6736(76)90524-9. [DOI] [PubMed] [Google Scholar]
  12. Rota T. R., Nichols R. L. Infection of cell cultures by trachoma agent: enhancement by DEAE-dextran. J Infect Dis. 1971 Oct;124(4):419–421. doi: 10.1093/infdis/124.4.419. [DOI] [PubMed] [Google Scholar]
  13. Sompolinsky D., Richmond S. Growth of Chlamydia trachomatis in McCoy cells treated with cytochalasin B. Appl Microbiol. 1974 Dec;28(6):912–914. doi: 10.1128/am.28.6.912-914.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. TANG F. F., CHANG H. L., HUANG Y. T., WANG K. C. Studies on the etiology of trachoma with special reference to isolation of the virus in chick embryo. Chin Med J. 1957 Jun;75(6):429–447. [PubMed] [Google Scholar]
  15. WEISS E., DRESSLER H. R. Centrifugation and Rickettsiae and viruses onto cells and its effect on infection. Proc Soc Exp Biol Med. 1960 Apr;103:691–695. doi: 10.3181/00379727-103-25637. [DOI] [PubMed] [Google Scholar]
  16. Wentworth B. B., Alexander E. R. Isolation of Chlamydia trachomatis by use of 5-iodo-2-deoxyuridine-treated cells. Appl Microbiol. 1974 May;27(5):912–916. doi: 10.1128/am.27.5.912-916.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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