Abstract
Early replicator subculturing of blood specimens after 4 to 16 h of incubation detected 135 of 217 (59%) of all positive cultures, including 56 of 64 (87%) Haemophilus influenzae type b, 7 of 22 (32%) Staphylococcus aureus, 19 of 20 (95%) pathogenic streptococci, and 20 of 24 (83%) Enterobacteriaceae. The mean time to detection of the common pediatric pathogens (36 h) was significantly less than that of contaminants (85 h) (P less than 0.001). Blind subculturing to differential media aided in the rapid identification of isolates and the detection of mixed cultures. In addition, a method of obtaining rapid susceptibilities of blood and body fluid isolates to selected antibiotics by blind subculturing to antibiotic-containing media was evaluated. Immediate susceptibility information was obtained for 214 of the 245 (87.3%) isolate-antibiotic combinations. There was complete correlation with a standard Kirby-Bauer reading for 94.9% of these observations. Replicator blood subculturing before 24 h of incubation results in early detection of the majority of pediatric pathogens. The inoculation of additional differential and antibiotic-containing media with each blind subculture aids in rapid identification of isolates and may give limited, but clinically important, immediate susceptibility information.
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- Barkin R. M., Todd J. K., Roe M. H. Rapid ampicillin susceptibility testing for Haemophilus influenzae. Am J Clin Pathol. 1977 Jan;67(1):100–103. doi: 10.1093/ajcp/67.1.100. [DOI] [PubMed] [Google Scholar]
- Barry A. L., Joyce L. J., Adams A. P., Benner E. J. Rapid determination of antimicrobial susceptibility for urgent clinical situations. Am J Clin Pathol. 1973 May;59(5):693–699. doi: 10.1093/ajcp/59.5.693. [DOI] [PubMed] [Google Scholar]
- Blazevic D. J., Stemper J. E., Matsen J. M. Comparison of macroscopic examination, routine gram stains, and routine subcultures in the initial detection of positive blood cultures. Appl Microbiol. 1974 Mar;27(3):537–539. doi: 10.1128/am.27.3.537-539.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall M., Warren E., Washington J. A., 2nd Detection of bacteremia with liquid media containing sodium polyanetholsulfonate. Appl Microbiol. 1974 Jan;27(1):187–191. doi: 10.1128/am.27.1.187-191.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kluge R. M. Accuracy of Kirby-Bauer susceptibility tests read at 4, 8, and 12 hours of incubation: comparison with readings at 18 to 20 hours. Antimicrob Agents Chemother. 1975 Aug;8(2):139–145. doi: 10.1128/aac.8.2.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ryan K. J., Needham G. M., Dunsmoor C. L., Sherris J. C. Stability of antibiotics and chemotherapeutics in agar plates. Appl Microbiol. 1970 Sep;20(3):447–451. doi: 10.1128/am.20.3.447-451.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thornsberry C., Kirven L. A. Ampicillin resistance in Haemophilus influenzae as determined by a rapid test for beta-lactamase production. Antimicrob Agents Chemother. 1974 Nov;6(5):653–654. doi: 10.1128/aac.6.5.653. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Todd J. K., Roe M. H. Rapid detection of bacteremia by an early subculture technic. Am J Clin Pathol. 1975 Nov;64(5):694–699. doi: 10.1093/ajcp/64.5.694. [DOI] [PubMed] [Google Scholar]
- Washington J. A., 2nd Comparison of two commercially available media for detection of bacteremia. Appl Microbiol. 1971 Oct;22(4):604–607. doi: 10.1128/am.22.4.604-607.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wegner D. L., Mathis C. R., Neblett T. R. Direct method to determine the antibiotic susceptibility of rapidly growing blood pathogens. Antimicrob Agents Chemother. 1976 May;9(5):861–862. doi: 10.1128/aac.9.5.861. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winchester P. D., Todd J. K., Roe M. H. Bacteremia in hospitalized children. Am J Dis Child. 1977 Jul;131(7):753–758. doi: 10.1001/archpedi.1977.02120200035009. [DOI] [PubMed] [Google Scholar]