Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1977 Nov;6(5):474–481. doi: 10.1128/jcm.6.5.474-481.1977

Identification of Neisseria by electron capture gas-liquid chromatography of metabolites in a chemically defined growth medium.

C D Morse, J B Brooks, D S Kellogg Jr
PMCID: PMC274800  PMID: 21889

Abstract

A dual-purpose study was carried out in an attempt to develop a rapid, sensitive method to identify Neisseria species by gas chromatography and to learn more about the metabolism of these organisms. Sixty-nine isolates of Neisseria were grown in a chemically defined fluid medium; the spent medium was extracted sequentially at pH 2 with diethyl ether and at pH 10 with chloroform. The pH 10 extracts were derivatized with heptafluorobutyric anhydride and analyzed by electron capture gas-liquid chromatography. The resulting spent culture medium electron capture gas-liquid chromatography profiles showed several qualitative and significant quantitative differences among the Neisseria species potentially useful in separating and identifying these organisms. Putrescine and cadaverine which were present in the spent culture medium of some Neisseria, including N. gonorrhoeae, were tentatively identified. Substituting carbohydrates for the chemically defined medium containing glucose in the base medium produced altered profiles with increased quantitative and qualitative differences.

Full text

PDF
474

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bovre K., Fuglesang J. E., Henriksen S. D. Neisseria elongata. Presentation of new isolates. Acta Pathol Microbiol Scand B Microbiol Immunol. 1972;80(6):919–922. doi: 10.1111/j.0365-5563.1973.tb00020.x. [DOI] [PubMed] [Google Scholar]
  2. Brooks J. B., Cherry W. B., Thacker L., Alley C. C. Analysis by gas chromatography of amines and nitrosamines produced in vivo and in vitro by Proteus mirabilis. J Infect Dis. 1972 Aug;126(2):143–153. doi: 10.1093/infdis/126.2.143. [DOI] [PubMed] [Google Scholar]
  3. Brooks J. B., Kellogg D. S., Alley C. C., Short H. B., Handsfield H. H., Huff B. Gas chromatography as a potential means of diagnosing arthritis. I. Differentiation between staphylococcal, streptococcal, gonococcal, and traumatic arthritis. J Infect Dis. 1974 Jun;129(6):660–668. doi: 10.1093/infdis/129.6.660. [DOI] [PubMed] [Google Scholar]
  4. Brooks J. B., Kellogg D. S., Thacker L., Turner E. M. Analysis by gas chromatography of fatty acids found in whole cultural extracts of Neisseria species. Can J Microbiol. 1971 Apr;17(4):531–543. doi: 10.1139/m71-088. [DOI] [PubMed] [Google Scholar]
  5. Brooks J. B., Kellogg D. S., Thacker L., Turner E. M. Analysis by gas chromatography of hydroxy acids produced by several species of Neisseria. Can J Microbiol. 1972 Feb;18(2):157–168. doi: 10.1139/m72-026. [DOI] [PubMed] [Google Scholar]
  6. Carifo K., Catlin B. W. Neisseria gonorrhoeae auxotyping: differentiation of clinical isolates based on growth responses on chemically defined media. Appl Microbiol. 1973 Sep;26(3):223–230. doi: 10.1128/am.26.3.223-230.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Carson L. A., Favero M. S., Bond W. W., Petersen N. J. Morphological, biochemical, and growth characteristics of pseudomonas cepacia from distilled water. Appl Microbiol. 1973 Mar;25(3):476–483. doi: 10.1128/am.25.3.476-483.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Catlin B. W. Nutritional profiles of Neisseria gonorrhoeae, Neisseria meningitidis, and Neisseria lactamica in chemically defined media and the use of growth requirements for gonococcal typing. J Infect Dis. 1973 Aug;128(2):178–194. doi: 10.1093/infdis/128.2.178. [DOI] [PubMed] [Google Scholar]
  9. Favero M. S., Carson L. A., Bond W. W., Petersen N. J. Pseudomonas aeruginosa: growth in distilled water from hospitals. Science. 1971 Aug 27;173(3999):836–838. doi: 10.1126/science.173.3999.836. [DOI] [PubMed] [Google Scholar]
  10. Frantz I. D. Growth Requirements of the Meningococcus. J Bacteriol. 1942 Jun;43(6):757–761. doi: 10.1128/jb.43.6.757-761.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hollis D. G., Wiggins G. L., Weaver R. E. Neisseria lactamicus sp. n., a lactose-fermenting species resembling Neisseria meningitidis. Appl Microbiol. 1969 Jan;17(1):71–77. doi: 10.1128/am.17.1.71-77.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hunter K. M., McVeigh I. Development of a chemically defined medium for growth of Neisseria gonorrhoeae. Antonie Van Leeuwenhoek. 1970;36(2):305–316. doi: 10.1007/BF02069032. [DOI] [PubMed] [Google Scholar]
  13. Kenny C. P., Ashton F. E., Diena B. B., Greenberg L. A chemically defined protein-free liquid medium for the cultivation of some species of Neisseria. Bull World Health Organ. 1967;37(4):569–573. [PMC free article] [PubMed] [Google Scholar]
  14. Kingsbury D. T. Relationship between sulfadiazine resistance and the failure to ferment maltose in Neisseria meningitidis. J Bacteriol. 1967 Sep;94(3):557–561. doi: 10.1128/jb.94.3.557-561.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lambert M. A., Hollis D. G., Moss C. W., Weaver R. E., Thomas M. L. Cellular fatty acids of nonpathogenic Neisseria. Can J Microbiol. 1971 Dec;17(12):1491–1502. doi: 10.1139/m71-239. [DOI] [PubMed] [Google Scholar]
  16. Lewis V. J., Weaver R. E., Hollis D. G. Fatty acid composition of Neisseria species as determined by gas chromatography. J Bacteriol. 1968 Jul;96(1):1–5. doi: 10.1128/jb.96.1.1-5.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Martin J. E., Jr, Lester A. Transgrow, a medium for transport and growth of Neisseria gonorrhoeae and Neisseria meningitidis. HSMHA Health Rep. 1971 Jan;86(1):30–33. [PMC free article] [PubMed] [Google Scholar]
  18. Morse C. D., Brooks J. B., Kellogg D. S., Jr Electron capture gas chromatographic detection of acethylmethylcarbinol produced by neisseria gonorrhoeae. J Clin Microbiol. 1976 Jan;3(1):34–41. doi: 10.1128/jcm.3.1.34-41.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Morse S. A., Stein S., Hines J. Glucose metabolism in Neisseria gonorrhoeae. J Bacteriol. 1974 Nov;120(2):702–714. doi: 10.1128/jb.120.2.702-714.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moss C. W., Kellogg D. S., Jr, Farshy D. C., Lambert M. A., Thayer J. D. Cellular fatty acids of pathogenic Neisseria. J Bacteriol. 1970 Oct;104(1):63–68. doi: 10.1128/jb.104.1.63-68.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. SCHERP H. W. Neisseria and neisserial infections. Annu Rev Microbiol. 1955;9:319–334. doi: 10.1146/annurev.mi.09.100155.001535. [DOI] [PubMed] [Google Scholar]
  22. Vandekerkove M., Faucon R., Audiffren P., Oddou A. Contribution à l'étude pratique du métabolisme des glucides par Neisseria intracellularis. V. Mise en evidence de l'acetyl-methyl-carbinol produit à partir du glucose. Med Trop (Mars) 1965 Jul-Aug;25(4):457–462. [PubMed] [Google Scholar]
  23. WHITE L. A., KELLOGG D. S., Jr NEISSERIA GONORRHOEAE IDENTIFICATION IN DIRECT SMEARS BY A FLUORESCENT ANTIBODY-COUNTERSTAIN METHOD. Appl Microbiol. 1965 Mar;13:171–174. doi: 10.1128/am.13.2.171-174.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES