Abstract
A growth detection method utilizing an automated apparatus capable of rapidly detecting bacterial growth by measuring changes of electrical impedance in bacteriological medium was utilized with "mock" blood cultures containing various gram-negative and gram-positive bacteria. Measurement of changes of electrical impedance was found to ba as accurate and comparable for time of growth detection as the radiometric method for detection of the same bacteria using mock blood cultures. In a limited clinical trial the use of the electrical impedance apparatus detected in 1 positive specimen from 40 clinical blood specimens as rapidly as by radiometric measurement. Both methods were considerably faster for detecting bacterial growth as compared with conventional culture methods. The selected species of gram-positive and -negative organisms tested were all detected by the electrical impedance method, including aerobes and anerobes. However, addition of 5% CO2 to the incubation atmosphere enhanced detection of gram-positive organisms.
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Selected References
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- Blazevic D. J., Stemper J. E., Matsen J. M. Comparison of macroscopic examination, routine gram stains, and routine subcultures in the initial detection of positive blood cultures. Appl Microbiol. 1974 Mar;27(3):537–539. doi: 10.1128/am.27.3.537-539.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DeBlanc H. J., Jr, DeLand F., Wagner H. N., Jr Automated radiometric detection of bacteria in 2,967 blood cultures. Appl Microbiol. 1971 Nov;22(5):846–849. doi: 10.1128/am.22.5.846-849.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellner P. D. System for inoculation of blood in the laboratory. Appl Microbiol. 1968 Dec;16(12):1892–1894. doi: 10.1128/am.16.12.1892-1894.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Renner E. D., Gatheridge L. A., Washington J. A., 2nd Evaluation of radiometric system for detecting bacteremia. Appl Microbiol. 1973 Sep;26(3):368–372. doi: 10.1128/am.26.3.368-372.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Strauss R. R., Throm R., Friedman H. Radiometric detection of bacteremia: requirement for terminal subcultures. J Clin Microbiol. 1977 Feb;5(2):145–148. doi: 10.1128/jcm.5.2.145-148.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]