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. 1977 Nov;6(5):507–510. doi: 10.1128/jcm.6.5.507-510.1977

Enzyme-linked immunosorbent assay for chlamydial antibodies.

V J Lewis, W L Thacker, S H Mitchell
PMCID: PMC274806  PMID: 72759

Abstract

An enzyme-linked immunosorbent assay (ELISA) detected chlamydial antibodies in human sera. The assay antigen produced in cell cultures infected with Chlamydia psittaci was Formalin-fixed to microplates. Single convalescent-phase sera positive for chlamydial antibodies by a complement-fixation test were positive at even higher dilutions by ELISA. Paired sera with diagnostic rises in complement-fixing antibody showed seroconversion by ELISA also. Control sera from persons with no history of chlamydial infection were negative by both tests. Sera from patients with psittacosis or lymphogranuloma venereum were ELISA positive, indicating that the assay with the antigen used in this study is genus specific rather than species specific.

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Selected References

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  1. BENEDICT A. A., O'BRIEN E. A passive hemagglutination reaction for psittacosis. J Immunol. 1958 Feb;80(2):94–99. [PubMed] [Google Scholar]
  2. Bartlett A., Bidwell D. E. Enzyme immunoassays for parasitic diseases. Trans R Soc Trop Med Hyg. 1976;70(2):98–106. doi: 10.1016/0035-9203(76)90163-2. [DOI] [PubMed] [Google Scholar]
  3. Carlsson H. E., Hurvell B., Lindberg A. A. Enzyme-linked immunosorbent assay (ELISA) for titration of antibodies against Brucella abortus and Yersinia enterocolitica. Acta Pathol Microbiol Scand C. 1976 Jun;84(3):168–176. doi: 10.1111/j.1699-0463.1976.tb00016.x. [DOI] [PubMed] [Google Scholar]
  4. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  5. Engvall E., Perlmann P. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes. J Immunol. 1972 Jul;109(1):129–135. [PubMed] [Google Scholar]
  6. Gerloff R. K., Watson R. O. The radioisotope precipitation test for psittacosis group antibody. Am J Ophthalmol. 1967 May;63(5 Suppl):1492–1498. doi: 10.1016/0002-9394(67)94137-2. [DOI] [PubMed] [Google Scholar]
  7. HAHON N., COOKE K. O. FLUORESCENT CELL-COUNTING NEUTRALIZATION TEST FOR PSITTACOSIS. J Bacteriol. 1965 Jun;89:1465–1471. doi: 10.1128/jb.89.6.1465-1471.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hanna L., Jawetz E., Nabli B., Hoshiwara I., Ostler B., Dawson C. Titration and typing of serum antibodies in TRIC infections by immunofluorescence. J Immunol. 1972 Jan;108(1):102–107. [PubMed] [Google Scholar]
  9. Holmgren J., Svennerholm A. M. Enzyme-linked immunosorbent assays for cholera serology. Infect Immun. 1973 May;7(5):759–763. doi: 10.1128/iai.7.5.759-763.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Juchau S. V., Linscott W. D., Schachter J., Jawetz E. Inhibition of antichlamydial IgM antibody by IgG antibody in immunofluorescence tests. J Immunol. 1972 Jun;108(6):1563–1569. [PubMed] [Google Scholar]
  11. Lewis V. J., Thacker W. L., Engelman H. M. Indirect hemagglutination test for chlamydial antibodies. Appl Microbiol. 1972 Jul;24(1):22–25. doi: 10.1128/am.24.1.22-25.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  13. Ruitenberg E. J., Steerenberg P. A., Brosi B. J., Buys J. Serodiagnosis of Trichinella spiralis infections in pigs by enzyme-linked immunosorbent assays. Bull World Health Organ. 1974;51(1):108–109. [PMC free article] [PubMed] [Google Scholar]
  14. Russell H., Facklam R. R., Edwards L. R. Enzyme-linked immunosorbent assay for streptococcal M protein antibodies. J Clin Microbiol. 1976 May;3(5):501–505. doi: 10.1128/jcm.3.5.501-505.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schachter J., Hill E. C., King E. B., Coleman V. R., Jones P., Meyer K. F. Chlamydial infection in women with cervical dysplasia. Am J Obstet Gynecol. 1975 Dec 1;123(7):753–757. doi: 10.1016/0002-9378(75)90501-3. [DOI] [PubMed] [Google Scholar]
  16. Voller A., Bidwell D. E. A simple method for detecting antibodies to rubella. Br J Exp Pathol. 1975 Aug;56(4):338–339. [PMC free article] [PubMed] [Google Scholar]
  17. Voller A., Bidwell D. E. Enzyme-immunoassays for antibodies in measles, cytomegalovirus infections and after rubella vaccination. Br J Exp Pathol. 1976 Apr;57(2):243–247. [PMC free article] [PubMed] [Google Scholar]
  18. Voller A., Bidwell D., Huldt G., Engvall E. A microplate method of enzyme-linked immunosorbent assay and its application to malaria. Bull World Health Organ. 1974;51(2):209–211. [PMC free article] [PubMed] [Google Scholar]
  19. Wang S. P., Grayston J. T., Alexander E. R., Holmes K. K. Simplified microimmunofluorescence test with trachoma-lymphogranuloma venereum (Chlamydia trachomatis) antigens for use as a screening test for antibody. J Clin Microbiol. 1975 Mar;1(3):250–255. doi: 10.1128/jcm.1.3.250-255.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

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