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. Author manuscript; available in PMC: 2009 Sep 22.
Published in final edited form as: Oncogene. 2009 May 18;28(26):2436–2445. doi: 10.1038/onc.2009.98

Figure 3.

Figure 3

Interactions between Pin1 and Akt proteins. (a) Immunoprecipitated Akt can be recognized by anti-MPM2 antibody and vice versa. (b) Co-immunoprecipitation of endogenous Akt and Pin1 proteins in breast cancer MDA-MB-468 cell lysate. Approximately 4 mg of whole cell lysate was used for each co-immunoprecipitation, and about 50 μg of whole cell lysate (WCL) was loaded as control. (c) Precipitation of Akt by (glutathione-S-transferase) GST-Pin1 fusion protein pull-down assay (right panel) and (d) gel staining of purified GST, GST-Pin1, and GST-Pin1 (W34A) fusion proteins (left panel). (e) Co-immunoprecipitation of Pin1 and Akt proteins in MDA-MB-468 cells in the presence or absence of insulin-like growth factor (IGF)-1 (50 ng/ml) for 2 h and with or without addition of PI3 K inhibitor LY294002 (LY, 20 μm). IP, immunoprecipitation; WB, western blot; MPM2, antibody against phosphorylated Ser/Thr-Pro motifs.