Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1977 Dec;6(6):633–638. doi: 10.1128/jcm.6.6.633-638.1977

Evaluation of anti-complement immunofluorescence test in cytomegalovirus infection.

N Rao, D T Waruszewski, J A Armstrong, R W Atchison, M Ho
PMCID: PMC274836  PMID: 201665

Abstract

The anti-complement immunofluorescence (ACIF) technique was evaluated for the diagnosis of human cytomegalovirus (CMV) infection in a group of sera derived from renal transplant recipients and donors by comparing it with the indirect immunofluorescence (FA) and complement fixation (CF) TESTS. The ACIF and FA tests yielded similar results. However, the ACIF test had a distinct advantage over the indirect FA test, since it eliminated the nonspecific cytoplasmic staining that may result in false positive readings in inexperienced hands. Both the indirect FA and ACIF tests were more sensitive than the CF test. In primary CMV infection, the FA and ACIF antibodies appeared earlier and had significantly higher titer than corresponding CF titers. This difference in titers was not seen in seropositive individuals who lacked overt infection. Our previously reported correlation between the seropositivity of the donor and CMV infection in seronegative recipients has been confirmed.

Full text

PDF
633

Images in this article

635Image
on p.635

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen H. K. Complement-fixing and neutralizing antibodies against cytomegalovirus strain AD 169 in sera from infants with cytomegalovirus infection. Scand J Infect Dis. 1969;1(3):141–144. [PubMed] [Google Scholar]
  2. Andersen H. K. Complement-fixing and virus-neutralizing antibodies in cytomegalovirus infection as measured against homologous and heterologous antigen. Acta Pathol Microbiol Scand B Microbiol Immunol. 1970;78(4):504–508. doi: 10.1111/j.1699-0463.1970.tb04334.x. [DOI] [PubMed] [Google Scholar]
  3. Andersen H. K. Cytomegalovirus neutralization by plaque reduction. Arch Gesamte Virusforsch. 1971;35(2):143–151. doi: 10.1007/BF01249705. [DOI] [PubMed] [Google Scholar]
  4. Andersen H. K., Godtfredsen A., Spencer E. S. Studies on the specificity of the complement fixation test in cytomegalovirus infections. With special reference to possible cross-reactions with other herpesvirus antigens. Scand J Infect Dis. 1971;3(3):183–187. doi: 10.3109/inf.1971.3.issue-3.01. [DOI] [PubMed] [Google Scholar]
  5. Atchison R. W., Ordonez J. V., Sather G. E., Hammon W. M. Fluorescent antibody, complement fixation method for detection of dengue viruses in mice. J Immunol. 1966 Jun;96(6):936–943. [PubMed] [Google Scholar]
  6. Bernstein M. T., Stewart J. A. Indirect hemagglutination test for detection of antibodies to Cytomegalovirus. Appl Microbiol. 1971 Jan;21(1):84–89. doi: 10.1128/am.21.1.84-89.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Betts R. F., George S. D., Rundell R. B., Freeman R. B., Douglas R. G., Jr Comparative activity of immunofluorescent antibody and complement-fixing antibody in cytomegalovirus infection. J Clin Microbiol. 1976 Aug;4(2):151–156. doi: 10.1128/jcm.4.2.151-156.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Chiang W. T., Wentworth B. B., Alexander E. R. The use of an immunofluorescence technique for the determination of antibodies to cytomegalovirus strains in human sera. J Immunol. 1970 Apr;104(4):992–999. [PubMed] [Google Scholar]
  9. Dreesman G. R., Benyesh-Melnick M. Spectrum of human cytomegalovirus complement-fixing antigens. J Immunol. 1967 Dec;99(6):1106–1114. [PubMed] [Google Scholar]
  10. Furukawa T., Hornberger E., Sakuma S., Plotkin S. A. Demonstration of immunoglobulin G receptors induced by human cytomegalovirus. J Clin Microbiol. 1975 Oct;2(4):332–336. doi: 10.1128/jcm.2.4.332-336.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. GOLDWASSER R. A., SHEPARD C. C. Staining of complement and modification of fluorescent antibody procedures. J Immunol. 1958 Feb;80(2):122–131. [PubMed] [Google Scholar]
  12. Geder L. Evidence for early nuclear antigens in cytomegalovirus-infected cells. J Gen Virol. 1976 Aug;32(2):315–319. doi: 10.1099/0022-1317-32-2-315. [DOI] [PubMed] [Google Scholar]
  13. HINUMA Y., HUMMELER K. Studies on the complement-fixing antigens of poliomyelitis. III. Intracellular development of antigen. J Immunol. 1961 Oct;87:367–375. [PubMed] [Google Scholar]
  14. HINUMA Y., OHTA R., MIYAMOTO T., ISHIDA N. Evaluation of the complement method of fluorescent antibody technique with myxoviruses. J Immunol. 1962 Jul;89:19–26. [PubMed] [Google Scholar]
  15. Henle W., Guerra A., Henle G. False negative and prozone reactions in tests for antibodies to Epstein-Barr virus-associated nuclear antigen. Int J Cancer. 1974 Jun 15;13(6):751–754. doi: 10.1002/ijc.2910130603. [DOI] [PubMed] [Google Scholar]
  16. Ho M., Suwansirikul S., Dowling J. N., Youngblood L. A., Armstrong J. A. The transplanted kidney as a source of cytomegalovirus infection. N Engl J Med. 1975 Nov 27;293(22):1109–1112. doi: 10.1056/NEJM197511272932201. [DOI] [PubMed] [Google Scholar]
  17. Keller R., Peitchel R., Goldman J. N., Goldman M. An IgG-Fc receptor induced in cytomegalovirus-infected human fibroblasts. J Immunol. 1976 Mar;116(3):772–777. [PubMed] [Google Scholar]
  18. Lang D. J., Noren B. Cytomegaloviremia following congenital infection. J Pediatr. 1968 Dec;73(6):812–819. doi: 10.1016/s0022-3476(68)80233-1. [DOI] [PubMed] [Google Scholar]
  19. Penttinen K., Käriäinen L., Myllylä G. Cytomegalovirus antibody assay by platelet aggregation. Arch Gesamte Virusforsch. 1970;29(2):189–194. doi: 10.1007/BF01249304. [DOI] [PubMed] [Google Scholar]
  20. Reedman B. M., Hilgers J., Hilgers F., Klein G. Immunofluorescence and anti-complement immunofluorescence absorption tests for quantitation of Epstein-Barr virus-associated antigens. Int J Cancer. 1975 Apr 15;15(4):566–571. doi: 10.1002/ijc.2910150406. [DOI] [PubMed] [Google Scholar]
  21. Reedman B. M., Klein G. Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. Int J Cancer. 1973 May;11(3):499–520. doi: 10.1002/ijc.2910110302. [DOI] [PubMed] [Google Scholar]
  22. SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
  23. Schmitz H., Haas R. Determination of different cytomegalovirus immunoglobulins (IgG, IgA, IgM) by immunofluorescence. Arch Gesamte Virusforsch. 1972;37(1):131–140. doi: 10.1007/BF01241158. [DOI] [PubMed] [Google Scholar]
  24. Spencer E. S., Andersen H. K. The development of immunofluorescent antibodies as compared with complement-fixing and virus-neutralizing antibodies in human cytomegalovirus infection. Scand J Infect Dis. 1972;4(2):109–112. doi: 10.3109/inf.1972.4.issue-2.08. [DOI] [PubMed] [Google Scholar]
  25. Stagno S., Reynolds D. W., Huang E. S., Thames S. D., Smith R. J., Alford C. A. Congenital cytomegalovirus infection. N Engl J Med. 1977 Jun 2;296(22):1254–1258. doi: 10.1056/NEJM197706022962203. [DOI] [PubMed] [Google Scholar]
  26. Stagno S., Reynolds D. W., Tsiantos A., Fuccillo D. A., Long W., Alford C. A. Comparative serial virologic and serologic studies of symptomatic and subclinical congenitally and natally acquired cytomegalovirus infections. J Infect Dis. 1975 Nov;132(5):568–577. doi: 10.1093/infdis/132.5.568. [DOI] [PubMed] [Google Scholar]
  27. Stagno S., Reynolds D., Tsiantos A., Fuccillo D. A., Smith R., Tiller M., Alford C. A., Jr Cervical cytomegalovirus excretion in pregnant and nonpregnant women: suppression in early gestation. J Infect Dis. 1975 May;131(5):522–527. doi: 10.1093/infdis/131.5.522. [DOI] [PubMed] [Google Scholar]
  28. TAKATSY G. The use of spiral loops in serological and virological micro-methods. Acta Microbiol Acad Sci Hung. 1955;3(1-2):191–202. [PubMed] [Google Scholar]
  29. The T. H., Klein G., Langenhuysen M. M. Antibody reactions to virus-specific early antigens (EA) in patients with cytomegalovirus (CMV) infection. Clin Exp Immunol. 1974 Jan;16(1):1-7,9-12. [PMC free article] [PubMed] [Google Scholar]
  30. WELLER T. H., HANSHAW J. B. Virologic and clinical observations on cytomegalic inclusion disease. N Engl J Med. 1962 Jun 14;266:1233–1244. doi: 10.1056/NEJM196206142662401. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES