Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1978 Jan;7(1):59–62. doi: 10.1128/jcm.7.1.59-62.1978

Quantitative evaluation of three commercial blood culture media for growth of anaerobic organisms.

J I Mangels, L H Lindberg, K L Vosti
PMCID: PMC274857  PMID: 24059

Abstract

The ability of three different commercial blood culture media--brain heart infusion broth (Pfizer), thiol broth (Difco), and PRS-peptone broth (Becton, Dickinson & Co.)--to support the growth of five different anaerobes is described. Inocula of 100 and 1,000 colony-forming units per ml were used to evaluate potential differences in survival, lag time, growth rate, and doubling times of each anaerobe in each medium. In addition, each medium was evaluated for its ability to neutralize the antibacterial effects of whole blood. The results of this study indicate that the PRS-peptone broth is superior to brain heart infusion and thiol broths. Shorter lag times and accelerated generation times and growth rates were noted for more different anaerobes in the PRS-peptone broth. Neither the size of inoculum nor the addition of normal whole blood appeared to alter the survival or growth characteristics of the anaerobes in any medium. However, the addition of normal whole blood did extend the lag time of each anaerobe by approximately 1 to 2 h in each medium.

Full text

PDF
59

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BAUCHOP T., ELSDEN S. R. The growth of micro-organisms in relation to their energy supply. J Gen Microbiol. 1960 Dec;23:457–469. doi: 10.1099/00221287-23-3-457. [DOI] [PubMed] [Google Scholar]
  2. Finegold S. M., White M. L., Ziment I., Winn W. R. Rapid diagnosis of bacteremia. Appl Microbiol. 1969 Sep;18(3):458–463. doi: 10.1128/am.18.3.458-463.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. GIBBONS R. J., MACDONALD J. B. Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus. J Bacteriol. 1960 Aug;80:164–170. doi: 10.1128/jb.80.2.164-170.1960. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Graves M. H., Morello J. A., Kocka F. E. Sodium polyanethol sulfonate sensitivity of anaerobic cocci. Appl Microbiol. 1974 Jun;27(6):1131–1133. doi: 10.1128/am.27.6.1131-1133.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mangels J. I., Lindberg L. H., Vosti K. L. Comparative evaluation of three different commercial blood culture media for recovery of anaerobic organisms. J Clin Microbiol. 1977 May;5(5):505–509. doi: 10.1128/jcm.5.5.505-509.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morello J. A., Ellner P. D. New medium for blood cultures. Appl Microbiol. 1969 Jan;17(1):68–70. doi: 10.1128/am.17.1.68-70.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Rosner R. A quantitative evaluation of three blood culture systems. Am J Clin Pathol. 1972 Feb;57(2):220–227. doi: 10.1093/ajcp/57.2.220. [DOI] [PubMed] [Google Scholar]
  8. Rosner R. Evaluation of four blood culture systems using parallel culture methods. Appl Microbiol. 1974 Aug;28(2):245–247. doi: 10.1128/am.28.2.245-247.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stalons D. R., Thornsberry C., Dowell V. R., Jr Effect of culture medium and carbon dioxide concentration on growth of anaerobic bacteria commonly encountered in clinical specimens. Appl Microbiol. 1974 Jun;27(6):1098–1104. doi: 10.1128/am.27.6.1098-1104.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sullivan N. M., Sutter V. L., Carter W. T., Attebery H. R., Finegold S. M. Bacteremia after genitourinary tract manipulation: bacteriological aspects and evaluation of various blood culture systems. Appl Microbiol. 1972 Jun;23(6):1101–1106. doi: 10.1128/am.23.6.1101-1106.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Washington J. A., 2nd Comparison of two commercially available media for detection of bacteremia. Appl Microbiol. 1971 Oct;22(4):604–607. doi: 10.1128/am.22.4.604-607.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Washington J. A., 2nd Evaluation of two commercially available media for detection of bacteremia. Appl Microbiol. 1972 May;23(5):956–959. doi: 10.1128/am.23.5.956-959.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Washington J. A., 2nd, Martin W. J. Comparison of three blood culture media for recovery of anaerobic bacteria. Appl Microbiol. 1973 Jan;25(1):70–71. doi: 10.1128/am.25.1.70-71.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wilson W. R., Martin W. J., Wilkowske C. J., Washington J. A., 2nd Anaerobic bacteremia. Mayo Clin Proc. 1972 Sep;47(9):639–646. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES