Abstract
A micromethod was developed for quantitative estimation in saliva of Streptococcus mutans and lactobacilli. With a semiautomatic pipette, 25 microliter of diluted saliva was spotted on the surface of an agar plate containing a selective medium. This volume gave a spot with a diameter of about 10 mm in which separate colonies could be counted. The results obtained with the spotting technique showed excellent agreement with those obtained with conventional agar plating. The method is convenient and results in a substantial saving of culture media.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bousfield I. J., Smith G. L., Trueman R. W. The use of semi-automatic pipettes in the viable counting of bacteria. J Appl Bacteriol. 1973 Jun;36(2):297–299. doi: 10.1111/j.1365-2672.1973.tb04105.x. [DOI] [PubMed] [Google Scholar]
- Gibbons R. J., van Houte J. Dental caries. Annu Rev Med. 1975;26:121–136. doi: 10.1146/annurev.me.26.020175.001005. [DOI] [PubMed] [Google Scholar]
- Gold O. G., Jordan H. V., Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol. 1973 Nov;18(11):1357–1364. doi: 10.1016/0003-9969(73)90109-x. [DOI] [PubMed] [Google Scholar]
- Jordan H. V., Krasse B., Möller A. A method of sampling human dental plaque for certain "caries-inducing" streptococci. Arch Oral Biol. 1968 Aug;13(8):919–927. doi: 10.1016/0003-9969(68)90007-1. [DOI] [PubMed] [Google Scholar]
- Larmas M. A new dip-slide method for the counting of salivary lactobacilli. Proc Finn Dent Soc. 1975 Apr;71(2):31–35. [PubMed] [Google Scholar]
- ROGOSA M., MITCHELL J. A., WISEMAN R. F. A selective medium for the isolation and enumeration of oral lactobacilli. J Dent Res. 1951 Oct;30(5):682–689. doi: 10.1177/00220345510300051201. [DOI] [PubMed] [Google Scholar]
- Scherp H. W. Dental caries: prospects for prevention. Science. 1971 Sep 24;173(4003):1199–1205. doi: 10.1126/science.173.4003.1199. [DOI] [PubMed] [Google Scholar]