Abstract
The immune adherence hemagglutination (IAHA) test has been used successfully to detect antibody to herpes simplex (HS) virus and varicella-zoster (V-Z) virus. Comparative studies between the complement-fixation (CF) test and the IAHA test revealed that, in most cases, the IAHA test was more sensitive than the CF test. Furthermore, diagnosis on the basis of a fourfold change in antibody titer was made more rapidly by the IAHA test. The IAHA test was found to be a very simple and practical technique requiring only a few hours for completion compared with the conventional CF test which required up to 24 h. In addition, both sera and cerebrospinal fluids could be tested in very low dilutions in the IAHA test, so that very low levels of antibody could be detected. Also, the IAHA test detected antibody to V-Z virus more frequently than did the CF test in adults with a history of varicella occurring 9 to 30 years prior to sampling. The level of cross-reaction between HS and V-Z viruses was examined by both the CF test and the IAHA test, and no major differences between the two techniques were found.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dienstag J. L., Cline W. L., Purcell R. H. Detection of cytomegalovirus antibody by immune adherence hemagglutination. Proc Soc Exp Biol Med. 1976 Dec;153(3):543–548. doi: 10.3181/00379727-153-39588. [DOI] [PubMed] [Google Scholar]
- Gershon A. A., Kalter Z. G., Steinberg S., Kuhns W. J. Detection of antibody to Varicella-Zoster virus by immune adherence hemagglutination. Proc Soc Exp Biol Med. 1976 Apr;151(4):762–765. doi: 10.3181/00379727-151-39302. [DOI] [PubMed] [Google Scholar]
- Ito M., Tagaya I. Immune adherence hemagglutination test as a new sensitive method for titration of animal virus antigens and antibodies. Jpn J Med Sci Biol. 1966 Jun;19(3):109–126. doi: 10.7883/yoken1952.19.109. [DOI] [PubMed] [Google Scholar]
- Krugman S., Friedman H., Lattimer C. Viral hepatitis, type A. Identification by specific complement fixation and immune adherence tests. N Engl J Med. 1975 May 29;292(22):1141–1143. doi: 10.1056/NEJM197505292922201. [DOI] [PubMed] [Google Scholar]
- Mayumi M., Okochi K., Nishioka K. Detection of Australia antigen by means of immune adherence haemagglutination test. Vox Sang. 1971 Feb;20(2):178–181. doi: 10.1111/j.1423-0410.1971.tb00549.x. [DOI] [PubMed] [Google Scholar]
- Miller W. J., Provost P. J., McAleer W. J., Ittensohn O. L., Villarejos V. M., Hilleman M. R. Specific immune adherence assay for human hepatitis A antibody application to diagnostic and epidemiologic investigations. Proc Soc Exp Biol Med. 1975 May;149(1):254–261. doi: 10.3181/00379727-149-38783. [DOI] [PubMed] [Google Scholar]
- Moritsugu Y., Dienstag J. L., Valdesuso J., Wong D. C., Wagner J., Routenberg J. A., Purcell R. H. Purification of hepatitis A antigen from feces and detection of antigen and antibody by immune adherence hemagglutination. Infect Immun. 1976 Mar;13(3):898–908. doi: 10.1128/iai.13.3.898-908.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NELSON R. A., Jr The immune-adherence phenomenon; an immunologically specific reaction between microorganisms and erythrocytes leading to enhanced phagocytosis. Science. 1953 Dec 18;118(3077):733–737. doi: 10.1126/science.118.3077.733. [DOI] [PubMed] [Google Scholar]
- SEVER J. L. Application of a microtechnique to viral serological investigations. J Immunol. 1962 Mar;88:320–329. [PubMed] [Google Scholar]
- Tsuda F., Takahashi T., Takahashi K., Miyakawa Y., Mayumi M. Determination of antibody to hepatitis B core antigen by means of immune adherence hemagglutination. J Immunol. 1975 Sep;115(3):834–838. [PubMed] [Google Scholar]
- Zissis G., Clinet G. Letter: Viral-antibody detection by a more sensitive complement-fixation reaction. Lancet. 1974 Apr 20;1(7860):754–755. doi: 10.1016/s0140-6736(74)92982-1. [DOI] [PubMed] [Google Scholar]