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. 1978 Feb;7(2):127–132. doi: 10.1128/jcm.7.2.127-132.1978

Bromothymol blue broth: improved medium for detection of Ureaplasma urealyticum (T-strain mycoplasma).

J A Robertson
PMCID: PMC274878  PMID: 632344

Abstract

Bromothymol blue (B) broth for the cultivation, detection, and identification of Ureaplasma urealyticum is described. In this medium, strains Cook and 960 had shorter generation times (60 min or less) and reached higher populations (over 10(8)) than have yet been reported for this species. Furthermore, the indicator changes color before the end of logarithmic growth, and the cultures retain viability for at least 1 day thereafter, greatly simplifying the handling of the organism. When the populations in cultures of these two strains and seven new isolates were determined, growth was detected earlier and proceeded to higher final titers in B broth than in urease test color medium (U-9 broth). The inclusion of antibiotics in B broth for use in clinical laboratories (B/NL broth) made the medium selective, specific, and more sensitive for the isolation of U. urealyticum. Comparison of B/NL broth with genital mycoplasma (GM) agar and U-9 broth for the primary isolation of U. urealyticum was made with 183 urethral swabs. All 70 isolates were detected on B/NL broth, but only 66 and 63 isolates were detected on GM agar and in U-9 broth, respectively. Moreover, the cultures in B/NL broth were pure and at titers that generally showed good correlation with colony counts on GM agar.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ajello F., Romano N. Effect of L-histidine on the survival of a T-strain of mycoplasma. Appl Microbiol. 1975 Feb;29(2):293–294. doi: 10.1128/am.29.2.293-294.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braun P., Klein J. O., Kass E. H. Susceptibility of genital mycoplasmas to antimicrobial agents. Appl Microbiol. 1970 Jan;19(1):62–70. doi: 10.1128/am.19.1.62-70.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brighton W. D., Windsor G. D., Andrews B. E., Williams R. E. Continuous culture of Mycoplasma species. Mon Bull Minist Health Public Health Lab Serv. 1967 Aug;26:154–158. [PubMed] [Google Scholar]
  4. CLYDE W. A., Jr MYCOPLASMA SPECIES IDENTIFICATION BASED UPON GROWTH INHIBITION BY SPECIFIC ANTISERA. J Immunol. 1964 Jun;92:958–965. [PubMed] [Google Scholar]
  5. Csonka G., Corse J. Selective inhibition in vitro of Mycoplasma hominis by lincomycin. Br J Vener Dis. 1970 Jun;46(3):203–204. doi: 10.1136/sti.46.3.203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ford D. K., MacDonald J. Influence of urea on the growth of T-strain mycoplasmas. J Bacteriol. 1967 May;93(5):1509–1512. doi: 10.1128/jb.93.5.1509-1512.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Furness G. T-mycoplasmas: some factors affecting their growth, colonial morphology, and assay on agar. J Infect Dis. 1973 Dec;128(6):703–709. doi: 10.1093/infdis/128.6.703. [DOI] [PubMed] [Google Scholar]
  8. Hendley J. O., Allred E. N. Prolonged stationary-phase growth of T-strain mycoplasmas in broth culture. Infect Immun. 1972 Feb;5(2):164–168. doi: 10.1128/iai.5.2.164-168.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kenny G. E., Cartwright F. D. Effect of urea concentration on growth of Ureaplasma urealyticum (T-strain mycoplasma). J Bacteriol. 1977 Oct;132(1):144–150. doi: 10.1128/jb.132.1.144-150.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kundsin R. B., Driscoll S. G., Ming P. L. Strain of mycoplasma associated with human reproductive failure. Science. 1967 Sep 29;157(3796):1573–1574. doi: 10.1126/science.157.3796.1573. [DOI] [PubMed] [Google Scholar]
  11. Masover G. K., Benson J. R., Hayflick L. Growth of T-strain mycoplasmas in medium without added urea: effect of trace amounts of urea and of a urease inhibitor. J Bacteriol. 1974 Feb;117(2):765–774. doi: 10.1128/jb.117.2.765-774.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Masover G. K., Catlin H., Hayflick L. The effect of growth and urea concentration on ammonia production by a urea-hydrolysing mycoplasma (Ureaplasma urealyticum). J Gen Microbiol. 1977 Feb;98(2):587–593. doi: 10.1099/00221287-98-2-587. [DOI] [PubMed] [Google Scholar]
  13. Masover G. K., Hayflick L. Dialysis culture of T-strain mycoplasmas. J Bacteriol. 1974 Apr;118(1):46–52. doi: 10.1128/jb.118.1.46-52.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Masover G. K., Razin S., Hayflick L. Localization of enzymes in Ureaplasma urealyticum (T-strain mycoplasma). J Bacteriol. 1977 Apr;130(1):297–302. doi: 10.1128/jb.130.1.297-302.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pickart L., Thaler M. M. Tripeptide in human serum which prolongs survival of normal liver cells and stimulates growth in neoplastic liver. Nat New Biol. 1973 May 16;243(124):85–87. [PubMed] [Google Scholar]
  16. Robertson J., Gomersall M., Gill P. Mycoplasma hominis: growth, reproduction, and isolation of small viable cells. J Bacteriol. 1975 Nov;124(2):1007–1018. doi: 10.1128/jb.124.2.1007-1018.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. SHEPARD M. C. T-Form colonies of pleuropneumonialike organisms. J Bacteriol. 1956 Mar;71(3):362–369. doi: 10.1128/jb.71.3.362-369.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shepard M. C. Differential methods for identification of T-mycoplasmas based on demonstration of urease. J Infect Dis. 1973 Mar;127(Suppl):S22–S25. doi: 10.1093/infdis/127.supplement_1.s22. [DOI] [PubMed] [Google Scholar]
  19. Shepard M. C., Lunceford C. D. Occurrence of urease in T strains of Mycoplasma. J Bacteriol. 1967 May;93(5):1513–1520. doi: 10.1128/jb.93.5.1513-1520.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Shepard M. C., Lunceford C. D. Urease color test medium U-9 for the detection and identification of "T" mycoplasms in clinical material. Appl Microbiol. 1970 Oct;20(4):539–543. doi: 10.1128/am.20.4.539-543.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Windsor G. D., Trigwell J. A. Prolonged survival of Ureaplasma urealyticum in liquid culture. J Med Microbiol. 1976 Feb;9(1):101–103. doi: 10.1099/00222615-9-1-101. [DOI] [PubMed] [Google Scholar]

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