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. 1978 Feb;7(2):172–175. doi: 10.1128/jcm.7.2.172-175.1978

In vitro stimulation of bovine peripheral blood lymphocytes: comparison of round- and flat-bottom microtiter plates for detection of tuberculin hypersensitivity.

E D Sloane, C C Muscoplat, J M Kaneene, D J Klausner, C O Thoen, D W Johnson
PMCID: PMC274887  PMID: 632347

Abstract

Lymphocytes from Mycobacterium bovis-sensitized and normal cattle were cultured in round- and/or flat-bottom microtiter plates and stimulated with M. bovis purified protein derivative (PPD) tuberculin. Blastogenic responses of lymphocytes from M. bovis-sensitized cattle to PPD cultured in round-bottom plates were significantly greater than those of lymphocytes cultured in flat-bottom microtiter plates. Normal lymphocytes of nonsensitized cattle were not stimulated by PPD in either round- or flat-bottom microtiter plates. Kinetics of lymphocyte responses in round-bottom plates are presented.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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