Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1978 Feb;7(2):180–183. doi: 10.1128/jcm.7.2.180-183.1978

Fermentation of raffinose by lactose-fermenting strains of Yersinia enterocolitica and by sucrose-fermenting strains of Escherichia coli.

G Cornelis, R K Luke, M H Richmond
PMCID: PMC274889  PMID: 344338

Abstract

Introduction of plasmids carrying the lacY gene (lactose permease gene) into Yersinia enterocolitica results in cells being able to ferment both lactose and raffinose. Transfer of such plasmids into Escherichia coli C600 (lacY) confers ability to ferment lactose but not raffinose. Derivatives of C600 that ferment both lactose and sucrose (Lac+ Scr+ strains) are able to ferment raffinose, but do not grow well on raffinose minimal medium. Fermentation of raffinose by Lac+ strains of Y. enterocolitica, and by Lac+ Scr+ strains of E. coli, is explained in terms of transport of raffinose via the lac permease and subsequent breakdown catalyzed by invertase.

Full text

PDF
180

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Achtman M., Willetts N., Clark A. J. Beginning a genetic analysis of conjugational transfer determined by the F factor in Escherichia coli by isolation and characterization of transfer-deficient mutants. J Bacteriol. 1971 May;106(2):529–538. doi: 10.1128/jb.106.2.529-538.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arditti R. R., Scaife J. G., Beckwith J. R. The nature of mutants in the lac promoter region. J Mol Biol. 1968 Dec;38(3):421–426. doi: 10.1016/0022-2836(68)90396-3. [DOI] [PubMed] [Google Scholar]
  3. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Burstein C., Kepes A. The alpha-galactosidase from Escherichia coli K12. Biochim Biophys Acta. 1971 Jan 26;230(1):52–63. doi: 10.1016/0304-4165(71)90053-5. [DOI] [PubMed] [Google Scholar]
  5. Cornelis G., Bennett P. M., Grinsted J. Properties of pGC1, a lac plasmid orginating in Yersinia enterocolitica 842. J Bacteriol. 1976 Sep;127(3):1058–1062. doi: 10.1128/jb.127.3.1058-1062.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cornelis G., Colson C. Restriction of DNA in Yersinia enterocolitica detected by recipient ability for a derepressed R factor from Escherichia coli. J Gen Microbiol. 1975 Apr;87(2):285–291. doi: 10.1099/00221287-87-2-285. [DOI] [PubMed] [Google Scholar]
  7. Grinsted J., Saunders J. R., Ingram L. C., Sykes R. B., Richmond M. H. Properties of a R factor which originated in Pseudomonas aeruginosa 1822. J Bacteriol. 1972 May;110(2):529–537. doi: 10.1128/jb.110.2.529-537.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guiso N., Ullmann A. Expression and regulation of lactose genes carried by plasmids. J Bacteriol. 1976 Aug;127(2):691–697. doi: 10.1128/jb.127.2.691-697.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Le Minor L., Coynault C., Rohde R., Rowe B., Aleksic S. Localisation plasmidique du déterminant génétique du caractète atypique "saccharose plus" des Salmonella. Ann Microbiol (Paris) 1973 Oct;124(3):295–306. [PubMed] [Google Scholar]
  10. MOLLARET H. H., CHEVALIER A. CONTRIBUTION 'A L''ETUDE D'UN NOUVEAU GROUPE DE GERMES PROCHES DU BACILLE DE MALASSEZ ET VIGNAL. I. CARACT'ERES CULTURAUX ET BIOCHIMIQUES. Ann Inst Pasteur (Paris) 1964 Jul;107:121–127. [PubMed] [Google Scholar]
  11. MOLLARET H. H., LUCAS A. SUR LES PARTICULARIT'ES BIOCHIMIQUES DES SOUCHES DE YERSINIA ENTEROCOLITICA ISOL'EES CHEZ LES LI'EVRES. Ann Inst Pasteur (Paris) 1965 Jan;108:121–125. [PubMed] [Google Scholar]
  12. Schmid K., Schmitt R. Raffinose metabolism in Escherichia coli K12. Purification and properties of a new alpha-galactosidase specified by a transmissible plasmid. Eur J Biochem. 1976 Aug 1;67(1):95–104. doi: 10.1111/j.1432-1033.1976.tb10637.x. [DOI] [PubMed] [Google Scholar]
  13. Schmitt R. Analysis of melibiose mutants deficient in alpha-galactosidase and thiomethylgalactoside permease II in Escherichia coli K-12. J Bacteriol. 1968 Aug;96(2):462–471. doi: 10.1128/jb.96.2.462-471.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Smith H. W., Parsell Z. Transmissible substrate-utilizing ability in enterobacteria. J Gen Microbiol. 1975 Mar;87(1):129–140. doi: 10.1099/00221287-87-1-129. [DOI] [PubMed] [Google Scholar]
  15. Wohlhieter J. A., Lazere J. R., Snellings N. J., Johnson E. M., Synenki R. M., Baron L. S. Characterization of transmissible genetic elements from sucrose-fermenting Salmonella strains. J Bacteriol. 1975 May;122(2):401–406. doi: 10.1128/jb.122.2.401-406.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES