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. 1978 Apr;7(4):323–326. doi: 10.1128/jcm.7.4.323-326.1978

Method for Rapid Detection of Group B Streptococci by Coagglutination

Diane S Leland 1, Rene C Lachapelle 1, Frederick M Wlodarski 1
PMCID: PMC274953  PMID: 357439

Abstract

A quick and reliable technique for the identification of group B streptococci has been developed. The method requires no elaborate equipment or expensive reagents and can be used to detect the group B organisms in mixed broth cultures or to identify suspect colonies selected from agar plates. The method is a coagglutination technique in which 1 drop of specifically sensitized protein A-containing Staphylococcus aureus is mixed with 1 drop of supernatant of an actively growing culture. The soluble group-specific carbohydrate substance of the group B streptococci reacts with the staph particles to produce agglutination that is macroscopically readable. One colony of group B streptococci taken from an agar plate and inoculated into Todd-Hewitt broth will give a positive reaction within 6 h of incubation; with a larger inoculum, the positive reaction occurs within a shorter period. The method was applied for detection of group B streptococci in mixed broth cultures. In laboratory studies involving random mixtures of organisms, 59.3% of positive cultures were detected within the first 8 h of incubation, and 71.7% were found within 24 h. In clinical studies with mixed broth cultures grown directly from vaginal swabs, 78.6% of the positive cultures were detected within the first 8 h of incubation, and 92.9% were found within 24 h.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker C. J., Barrett F. F. Transmission of group B streptococci among parturient women and their neonates. J Pediatr. 1973 Dec;83(6):919–925. doi: 10.1016/s0022-3476(73)80524-4. [DOI] [PubMed] [Google Scholar]
  2. CURTIS S. N., KRAUSE R. M. ANTIGENIC RELATIONSHIPS BETWEEN GROUPS B AND G STREPTOCOCCI. J Exp Med. 1964 Oct 1;120:629–637. doi: 10.1084/jem.120.4.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Christensen P., Kahlmeter G., Jonsson S., Kronvall G. New method for the serological grouping of Streptococci with specific antibodies adsorbed to protein A-containing staphylococci. Infect Immun. 1973 Jun;7(6):881–885. doi: 10.1128/iai.7.6.881-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dajani A. S. Rapid identification of beta hemolytic streptococci by counterimmunoelectrophoresis. J Immunol. 1973 Jun;110(6):1702–1705. [PubMed] [Google Scholar]
  5. Darling C. L. Standardization and evaluation of the CAMP reaction for the prompt, presumptive identification of Streptococcus agalactiae (Lancefield group B) in clinical material. J Clin Microbiol. 1975 Feb;1(2):171–174. doi: 10.1128/jcm.1.2.171-174.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edwards E. A., Larson G. L. New method of grouping beta-hemolytic streptococci directly on sheep blood agar plates by coagglutination of specifically sensitized protein A-containing staphylococci. Appl Microbiol. 1974 Dec;28(6):972–976. doi: 10.1128/am.28.6.972-976.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hahn G., Nyberg I. Identification of streptococcal groups A,B,C, and G by slide co-agglutination of antibody-sensitized protein A-containing staphylococci. J Clin Microbiol. 1976 Jul;4(1):99–101. doi: 10.1128/jcm.4.1.99-101.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hey D. J., Hall R. T., Burry V. F., Thurn A. N. Neonatal infections caused by group B streptococci. Am J Obstet Gynecol. 1973 May 1;116(1):43–47. doi: 10.1016/0002-9378(73)90881-8. [DOI] [PubMed] [Google Scholar]
  9. Hill H. R., Riter M. E., Menge S. K., Johnson D. R., Matsen J. M. Rapid identification of group B streptococci by counterimmunoelectrophoresis. J Clin Microbiol. 1975 Feb;1(2):188–191. doi: 10.1128/jcm.1.2.188-191.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hryniewicz W., Heczko P. B., Lütticken R., Wannamaker L. W. Comparison of three methods for grouping streptococci. J Clin Microbiol. 1976 Jul;4(1):28–31. doi: 10.1128/jcm.4.1.28-31.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kronvall G. A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. J Med Microbiol. 1973 May;6(2):187–190. doi: 10.1099/00222615-6-2-187. [DOI] [PubMed] [Google Scholar]
  12. Merritt K., Treadwell T. L., Jacobs N. J. Rapid recognition of group B streptococci by pigment production and counterimmunoelectrophoresis. J Clin Microbiol. 1976 Mar;3(3):287–290. doi: 10.1128/jcm.3.3.287-290.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Romero R., Wilkinson H. W. Identification of group B streptococci by immunofluorescence staining. Appl Microbiol. 1974 Aug;28(2):199–204. doi: 10.1128/am.28.2.199-204.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rosner R. Laboratory evaluation of a rapid four-hour serological grouping of groups A,B,C, and G beta-streptococci by the Phadebact streptococcus test. J Clin Microbiol. 1977 Jul;6(1):23–26. doi: 10.1128/jcm.6.1.23-26.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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