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. 1978 Apr;7(4):368–371. doi: 10.1128/jcm.7.4.368-371.1978

Comparison of WI-38, MRC-5, and IMR-90 cell strains for isolation of viruses from clinical specimens.

H M Friedman, C Koropchak
PMCID: PMC274969  PMID: 211143

Abstract

With the diminishing supply of the human fetal lung WI-38 cell strain, a replacement for viral isolation is needed. Two candidates are the human fetal lung strains MRC-5 and IMR-90. A comparison of WI-38, MRC-5, and IMR-90 was performed to evaluate efficiency and speed of viral isolation, clarity of cytophatic effect, and ease of growing the cells. The inocula were clinical specimens rather than tissue culture-adapted isolates. Frozen samples of 46 specimens that had previously yielded an isolate on WI-38 were thawed and inoculated onto WI-38, MRC-5, and IMR-90 cells. In addition, 95 freshly taken clinical specimens uf undetermined infectivity were inoculated onto the cell strains. Viral recovery rates were similar on all three strains, as were the appearance and speed of onset of the cytophatic effect. MRC-5 and WI-38 cells remained healthy until generation 36, whereas IMR-90 cells went into crisis by generation 20. The longer life span of the MRC-5 cells makes them more suitable than IMR-90 cells to replace the WI-38 strain for routine use in viral diagnosis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. M., Beem M. O. Use of human diploid cell cultures for primary isolation of respiratory syncytial virus. Proc Soc Exp Biol Med. 1966 Jan;121(1):205–209. doi: 10.3181/00379727-121-30738. [DOI] [PubMed] [Google Scholar]
  2. Chanock R. M., Ludwig W., Heubner R. J., Cate T. R., Chu L. W. Immunization by selective infection with type 4 adenovirus grown in human diploid tissue cultures. I. Safety and lack of oncogenicity and tests for potency in volunteers. JAMA. 1966 Feb 7;195(6):445–452. [PubMed] [Google Scholar]
  3. Fenters J. D., Fordyce P. A., Gerin J. L., Holper J. C. Propagation of Rhinovirus on WI-38 Cell Monolayers in Rolling Bottles. Appl Microbiol. 1967 Nov;15(6):1460–1464. doi: 10.1128/am.15.6.1460-1464.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HAYFLICK L., MOORHEAD P. S. The serial cultivation of human diploid cell strains. Exp Cell Res. 1961 Dec;25:585–621. doi: 10.1016/0014-4827(61)90192-6. [DOI] [PubMed] [Google Scholar]
  5. Herrmann E. C., Jr New concepts and developments in applied diagnostic virology. Prog Med Virol. 1974;17(0):221–289. [PubMed] [Google Scholar]
  6. Herrmann E. C., Jr, Person D. A., Smith T. F. Experience in laboratory diagnosis of enterovirus infections in routine medical practice. Mayo Clin Proc. 1972 Aug;47(8):577–586. [PubMed] [Google Scholar]
  7. Herrmann E. C., Jr The usefullness of human fibroblast cell lines for the isolation of viruses. Am J Epidemiol. 1967 Mar;85(2):200–206. doi: 10.1093/oxfordjournals.aje.a120683. [DOI] [PubMed] [Google Scholar]
  8. Jacobs J. P., Jones C. M., Baille J. P. Characteristics of a human diploid cell designated MRC-5. Nature. 1970 Jul 11;227(5254):168–170. doi: 10.1038/227168a0. [DOI] [PubMed] [Google Scholar]
  9. Levine E. M. Mycoplasma contamination of animal cell cultures: a simple, rapid detection method. Exp Cell Res. 1972 Sep;74(1):99–109. doi: 10.1016/0014-4827(72)90484-3. [DOI] [PubMed] [Google Scholar]
  10. Nichols W. W., Murphy D. G., Cristofalo V. J., Toji L. H., Greene A. E., Dwight S. A. Characterization of a new human diploid cell strain, IMR-90. Science. 1977 Apr 1;196(4285):60–63. doi: 10.1126/science.841339. [DOI] [PubMed] [Google Scholar]
  11. Plotkin S. A., Cornfeld D., Ingalls T. H. Studies of immunization with living rubella virus. Trials in children with a strain cultured from an aborted fetus. Am J Dis Child. 1965 Oct;110(4):381–389. doi: 10.1001/archpedi.1965.02090030401007. [DOI] [PubMed] [Google Scholar]
  12. Plotkin S. A., Wiktor T. J., Koprowski H., Rosanoff E. I., Tint H. Immunization schedules for the new human diploid cell vaccine against rabies. Am J Epidemiol. 1976 Jan;103(1):75–80. doi: 10.1093/oxfordjournals.aje.a112207. [DOI] [PubMed] [Google Scholar]
  13. Wade N. Hayflick's Tragedy: The Rise and Fall of a Human Cell Line. Science. 1976 Apr 9;192(4235):125–127. doi: 10.1126/science.192.4235.125. [DOI] [PubMed] [Google Scholar]
  14. Yang J. P., Chiang W., Gale J. L., Chen N. S. A chick-embyo cell microtest for typing of Herpesvirus hominis (38531). Proc Soc Exp Biol Med. 1975 Feb;148(2):324–328. doi: 10.3181/00379727-148-38531. [DOI] [PubMed] [Google Scholar]

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