Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1978 Apr;7(4):392–393. doi: 10.1128/jcm.7.4.392-393.1978

Counterimmunoelectrophoresis for detection of microbial antigens: increased sensitivity with dextran-containing gels.

G R Siber, P Skapriwsky
PMCID: PMC274974  PMID: 357445

Abstract

The addition of 4% dextran with a mean molecular weight of 70,000 to counterimmunoelectrophoresis gels enhanced the clarity of precipitin lines and increased the sensitivity of the procedure two- to fourfold with a variety of microbial antigens.

Full text

PDF
392

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ceska M. Human growth hormone-anti-(growth hormone) immunoprecipitates in the presence of dextrans. Biochem J. 1969 Feb;111(4):607–608. doi: 10.1042/bj1110607. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Ceska M. Insulin-anti-insulin precipitates in the presence of various dextrans. Immunology. 1968 Dec;15(6):837–843. [PMC free article] [PubMed] [Google Scholar]
  3. Feigin R. D., Wong M., Shackelford P. G., Stechenberg B. W., Dunkle L. M., Kaplan S. Countercurrent immunoelectrophoresis of urine as well as of CSF and blood for diagnosis of bacterial meningitis. J Pediatr. 1976 Nov;89(5):773–775. doi: 10.1016/s0022-3476(76)80802-5. [DOI] [PubMed] [Google Scholar]
  4. Gocke D. J., Howe C. Rapid detection of Australia antigen by counterimmunoelectrophoresis. J Immunol. 1970 Apr;104(4):1031–1034. [PubMed] [Google Scholar]
  5. Hellsing K. Immune reactions in polysaccharide media. The composition of the antigen-antibody complexes in the precipitin reaction. Biochem J. 1969 Aug;114(1):141–144. doi: 10.1042/bj1140141. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ingram D. L., Anderson P., Smith D. H. Countercurrent immunoelectrophoresis in the diagnosis of systemic diseases caused by Hemophilus infleunzae type b. J Pediatr. 1972 Dec;81(6):1156–1159. doi: 10.1016/s0022-3476(72)80252-x. [DOI] [PubMed] [Google Scholar]
  7. Shackelford P. G., Campbell J., Feigin R. D. Countercurrent immunoelectrophoresis in the evaluation of childhood infections. J Pediatr. 1974 Oct;85(4):478–481. doi: 10.1016/s0022-3476(74)80448-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES