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. Author manuscript; available in PMC: 2009 Sep 23.
Published in final edited form as: Biochemistry. 2008 Jun 24;47(25):6711–6718. doi: 10.1021/bi800117f

Table 1.

Osmolyte exclusion from HPC at 5 and 20 °C

Osmolyte ΔNw,total 20 °C λ, Å 20 °C ΔNw,total 5 °C λ, Å 5 °C
Urea 0 - 0 -
Glycerol 9.7 3.25 20.3 3.45
Sorbitol 19.8 2.9 32.7 3.5
TMAO 14.7 2.2 17.5 1.95
Proline 19.9 2.9 27.1 3.3
Glycine Betaine 21.0 3.1 26.9 3.2

The total of excess water per glucose monomer is calculated by integrating the curves shown in Figure 2 from 12.6 Å to ∞ as prescribed in equation (3) assuming that Πexcess/Π0 = 1 is the maximum excess pressure. The error is ~10% for the decay length λ and ~15% for ΔNw.