Abstract
Antibody titers to cytomegalovirus were determined in 204 single-serum specimens and in 138 serum pairs, utilizing as antigen extracts of infected cells prepared by either freezing and thawing cells (FT antigen) or extracting them with a glycine buffer (GE antigen). With the serum pairs, 40% of the early sera and 44% of the late sera showed a fourfold or higher antibody titer with the GE antigen compared with those with the FT antigen, whereas 39% of the single sera had a fourfold or higher titer with the GE antigen. Sucrose density gradient centrifugation of the two antigen preparations indicated that the GE antigen contained two peaks of complement-fixing activity, one at a density of 1.18 to 1.22 g/cm3 and one at 1.06 to 1.10 g/cm3. The FT antigen had only one discernible peak of complement-fixing activity at 1.04 to 1.10 g/cm3. On electron microscopy, the more dense peak contained mainly nucleocapsids with some dense bodies and occasional enveloped virions, and the lighter peak was composed of amorphous material. Antibody titers obtained with the crude GE antigen and the more dense peak (designated viral antigen) were in agreement, whereas titers with the FT antigen agreed with those obtained with the lighter peak (soluble antigen). The antibody detected with both antigens was mainly immunoglobulin G.
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Selected References
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- Chambers R. W., Rose J. A., Rabson A. S., Bond H. E., Hall W. T. Propagation and purification of high-titer human cytomegalovirus. Appl Microbiol. 1971 Nov;22(5):914–918. doi: 10.1128/am.22.5.914-918.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craighead J. E., Kanich R. E., Almeida J. D. Nonviral microbodies with viral antigenicity produced in cytomegalovirus-infected cells. J Virol. 1972 Oct;10(4):766–775. doi: 10.1128/jvi.10.4.766-775.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hanshaw J. B. Congenital cytomegalovirus infection: laboratory methods of detection. J Pediatr. 1969 Dec;75(6):1179–1185. doi: 10.1016/s0022-3476(69)80373-2. [DOI] [PubMed] [Google Scholar]
- Krech U., Jung M., Sonnabend W. A study of complement-fixing, immunofluorescent, and neutralizing antibodies in human cytomegalovirus infections. Z Immunitatsforsch Exp Klin Immunol. 1971 Jun;141(5):411–429. [PubMed] [Google Scholar]
- Probstmeyer F., Benyesh-Melnick M., McCombs R. M. Comparison of human cytomegalovirus obtained by glycine extraction and by sonic disruption of infected cells. J Virol. 1967 Feb;1(1):241–243. doi: 10.1128/jvi.1.1.241-243.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]