Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1978 Jun;7(6):514–518. doi: 10.1128/jcm.7.6.514-518.1978

New medium for isolation of Actinomyces viscosus and Actinomyces naeslundii from dental plaque.

K S Kornman, W J Loesche
PMCID: PMC275053  PMID: 670376

Abstract

Metronidazole (10 microgram/ml) and cadmium sulfate (20 microgram/ml) were added to a gelatin-based medium to select for microaerophilic Actinomyces species from dental plaque samples. The new medium (GMC), when incubated anaerobically, allowed 98% recovery of seven pure cultures of Actinomyces viscosus and 73% recovery of eight pure cultures of Actinomyces naeslundii, while suppressing 76% of the total count of other organisms in dental plaque samples. In 203 plaque samples, recoveries of A. viscosus and A. naeslundii on GMC and another selective medium for oral Actinomyces (CNAC-20) were compared. Recovery of A. viscosus was comparable on the two media. Recovery of A. naeslundii was significantly higher on GMC than CNAC-20 (P is less than 0.001), and GMC allowed a more characteristic cell morphology of both organisms. GMC medium appears to be useful for the isolation and presumptive identification of A. viscosus and A. naeslundii from dental plaque.

Full text

PDF
514

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arank A., Syed S. A., Kenney E. B., Freter R. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol. 1969 Apr;17(4):568–576. doi: 10.1128/am.17.4.568-576.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Babich H., Stotzky G. Sensitivity of Various Bacteria, Including Actinomycetes, and Fungi to Cadmium and the Influence of pH on Sensitivity. Appl Environ Microbiol. 1977 Mar;33(3):681–695. doi: 10.1128/aem.33.3.681-695.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chow A. W., Patten V., Guze L. B. Susceptibility of anaerobic bacteria to metronidazole: relative resistance of non-spore-forming gram-positive baccilli. J Infect Dis. 1975 Feb;131(2):182–185. doi: 10.1093/infdis/131.2.182. [DOI] [PubMed] [Google Scholar]
  4. Edwards D. I., Dye M., Carne H. The selective toxicity of antimicrobial nitroheterocyclic drugs. J Gen Microbiol. 1973 May;76(1):135–145. doi: 10.1099/00221287-76-1-135. [DOI] [PubMed] [Google Scholar]
  5. Ellen R. P., Balcerzak-Raczkowski I. B. Differential medium for detecting dental plaque bacteria resembling Actinomyces viscosus and Actinomyces naeslundii. J Clin Microbiol. 1975 Oct;2(4):305–310. doi: 10.1128/jcm.2.4.305-310.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Goldstein B. P., Nielsen E., Berti M., Bolzoni G., Silvestri L. G. The mechanism of action of nitro-heterocyclic antimicrobial drugs. Primary target of 1-methyl-2-nitro-5-vinylimidazole is DNA. J Gen Microbiol. 1977 Jun;100(2):271–281. doi: 10.1099/00221287-100-2-271. [DOI] [PubMed] [Google Scholar]
  7. Goldstein B. P., Vidal-Plana R. R., Cavalleri B., Zerilli L., Carniti G., Silvestri L. G. The mechanism of action of nitro-heterocyclic antimicrobial drugs. Metabolic activation by micro-organisms. J Gen Microbiol. 1977 Jun;100(2):283–298. doi: 10.1099/00221287-100-2-283. [DOI] [PubMed] [Google Scholar]
  8. Irving J. T., Socransky S. S., Heeley J. D. Histological changes in experimental periodontal disease in gnotobiotic rats and conventional hamsters. J Periodontal Res. 1974;9(2):73–80. doi: 10.1111/j.1600-0765.1974.tb00656.x. [DOI] [PubMed] [Google Scholar]
  9. JORDAN H. V., KEYES P. H. AEROBIC, GRAM-POSITIVE, FILAMENTOUS BACTERIA AS ETIOLOGIC AGENTS OF EXPERIMENTAL PERIODONTAL DISEASE IN HAMSTERS. Arch Oral Biol. 1964 Jul-Aug;9:401–414. doi: 10.1016/0003-9969(64)90025-1. [DOI] [PubMed] [Google Scholar]
  10. Jordan H. V., Keyes P. H., Bellack S. Periodontal lesions in hamsters and gnotobiotic rats infected with actinomyces of human origin. J Periodontal Res. 1972;7(1):21–28. doi: 10.1111/j.1600-0765.1972.tb00627.x. [DOI] [PubMed] [Google Scholar]
  11. Lerner P. I. Susceptibility of pathogenic actinomycetes to antimicrobial compounds. Antimicrob Agents Chemother. 1974 Mar;5(3):302–309. doi: 10.1128/aac.5.3.302. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Loesche W. J., Hockett R. N., Syed S. A. The predominant cultivable flora of tooth surface plaque removed from institutionalized subjects. Arch Oral Biol. 1972 Sep;17(9):1311–1325. doi: 10.1016/0003-9969(72)90164-1. [DOI] [PubMed] [Google Scholar]
  13. Prince H. N., Grunberg E., Titsworth E., DeLorenzo W. F. Effects of l-(2-nitro-l-imidazolyl)-3-methoxy-2-propanol and 2-methyl-5-nitroimidazole-l-ethanol against anaerobic and aerobic bacteria and protozoa. Appl Microbiol. 1969 Nov;18(5):728–730. doi: 10.1128/am.18.5.728-730.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Slots J. Microflora in the healthy gingival sulcus in man. Scand J Dent Res. 1977 May;85(4):247–254. doi: 10.1111/j.1600-0722.1977.tb00560.x. [DOI] [PubMed] [Google Scholar]
  15. Syed S. A. A new medium for the detection of gelatin-hydrolyzing activity of human dental plaque flora. J Clin Microbiol. 1976 Feb;3(2):200–202. doi: 10.1128/jcm.3.2.200-202.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Syed S. A., Loesche W. J., Pape H. L., Jr, grenier E. Predominant cultivable flora isolated from human root surface caries plaque. Infect Immun. 1975 Apr;11(4):727–731. doi: 10.1128/iai.11.4.727-731.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tally F. P., Sutter V. L., Finegold S. M. Metronidazole versus anaerobes. In vitro data and initial clinical observations. Calif Med. 1972 Dec;117(6):22–26. [PMC free article] [PubMed] [Google Scholar]
  18. Videau D., Blanchard J. C., Sebald M. Flore bucco-dentaire. Sensibilité aux antibiotiques et intéret de l'association spiramycine-métronidazole. Ann Microbiol (Paris) 1973 Dec;124(4):505–516. [PubMed] [Google Scholar]
  19. Williams B. L., Pantalone R. M., Sherris J. C. Subgingival microflora and periodontitis. J Periodontal Res. 1976 Feb;11(1):1–18. doi: 10.1111/j.1600-0765.1976.tb00045.x. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES