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. 1975 Mar;1(3):311–317. doi: 10.1128/jcm.1.3.311-317.1975

Detection by radioimmunoassay of antibodies in human smallpox patients and vaccinees.

D W Ziegler, H D Hutchinson, J P Koplan, J H Nakano
PMCID: PMC275071  PMID: 170309

Abstract

A radioimmunoassay procedure was developed for determining smallpox and vaccinia antibodies in human sera. The test detected and measured both primary and secondary immune responses in persons infected with variola virus or vaccinia virus. The antibody titers obtained by complement fixation, hemagglutination inhibition, plaque reduction neutralization, and radioimmunoassay methods were compared. In sequential serum specimens, the radioimmunoassay test indicated fourfold or greater increases in all of the smallpox patients and in six of eight vaccinated persons. Both the complement fixation and the hemagglutination inhibition tests were less effective. In persons who had been vaccinated, radioimmunoassay and plaque reduction neutralization tests appeared to measure the same immune response. However, in smallpox patients the immune response was readily detected by radioimmunoassay, whereas an immune response was not detected by the plaque reduction neutralization test when vaccinia virus was the antigen in the test system. Radioimmunoassay is an operationally simple procedure which provides objective and quantitative end-point titers in serological determinations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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