Abstract
An immune electron microscopy agglutination technique in which emphasis is placed upon the importance of antigen-antibody equivalence has been developed as a possible method for the serotyping of avian infectious bronchitis viruses. The Connecticut and Massachusetts 41 serotypes were used as a model system. Stock virus concentrations were standardized by physical particle counts of virions sedimented directly onto electron microscope specimen grids. Suspensions containing approximately 150 virions per grid square were allowed to react with dilutions of homologous and heterologous antisera. Virions in these constant virus-variable serum mixtures were sedimented directly onto electron microscope specimen grids, and the relative degree of aggregation per grid was determined from the mean percent aggregation of five randomly selected grid squares. In homologous assays, regions of relative antibody excess, of equivalence, and of relative antigen excess were clearly evident. At equivalence, the mean percent aggregation was significantly higher than in the regions of relative antibody or antigen excess. In the heterologous systems, the degree of aggregation differed little from that of the virus controls containing no antiserum.
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- ALMEIDA J., CINADER B., HOWATSON A. THE STRUCTURE OF ANTIGEN-ANTIBODY COMPLEXES. A STUDY BY ELECTRON MICROSCOPY. J Exp Med. 1963 Sep 1;118:327–340. doi: 10.1084/jem.118.3.327. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Almeida J. D., Waterson A. P. The morphology of virus-antibody interaction. Adv Virus Res. 1969;15:307–338. doi: 10.1016/S0065-3527(08)60878-7. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berry D. M., Almeida J. D. The morphological and biological effects of various antisera on avian infectious bronchitis virus. J Gen Virol. 1968 Jul;3(1):97–102. doi: 10.1099/0022-1317-3-1-97. [DOI] [PubMed] [Google Scholar]
- Cunningham C. H. Avian infectious bronchitis. Adv Vet Sci Comp Med. 1970;14:105–148. [PubMed] [Google Scholar]
- Dienstag J. L., Alling D. W., Purcell R. H. Quantitation of antibody to hepatitis A antigen by immune electron microscopy. Infect Immun. 1976 Apr;13(4):1209–1213. doi: 10.1128/iai.13.4.1209-1213.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Dienstag J. L., Schulman A. N., Gerety R. J., Hoofnagle J. H., Lorenz D. E., Purcell R. H., Barker L. F. Hepatitis A antigen isolated from liver and stool: immunologic comparison of antisera prepared in guinea pigs. J Immunol. 1976 Sep;117(3):876–881. [PubMed] [Google Scholar]
- Hopkins S. R. Serological comparisons of strains of infectious bronchitis virus using plaque-purified isolants. Avian Dis. 1974 Apr-Jun;18(2):231–239. [PubMed] [Google Scholar]
- Johnson R. B., Marquardt W. W., Newman J. A. A new serotype of infectious bronchitis virus responsible for respiratory disease in Arkansas broiler flocks. Avian Dis. 1973 Jul-Sep;17(3):518–523. [PubMed] [Google Scholar]
- Johnson R. B., Marquardt W. W. The neutralizing characteristics of strains of infectious bronchitis virus as measured by the constant-virus variable-serum method in chicken tracheal cultures. Avian Dis. 1975 Jan-Mar;19(1):82–90. [PubMed] [Google Scholar]
- Johnson R. B., Newman J. A., Wills F. K. Titration of infectious bronchitis virus in tracheas of susceptible and immune chickens. Avian Dis. 1969 Aug;13(3):632–635. [PubMed] [Google Scholar]