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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1975 Apr;1(4):366–376. doi: 10.1128/jcm.1.4.366-376.1975

New human adenovirus isolated from a renal transplant recipient: description and characterization of candiate adenovirus type 34.

J C Hierholzer, N O Atuk, J M Gwaltney Jr
PMCID: PMC275094  PMID: 170313

Abstract

An antigenically distinct adenovirus is described which was isolated in March 1972 from the urine of a 17-year-old Caucasian male who was experiencing fever after receiving a kidney transplant from a cadaver in February. The adenovirus could not be isolated in April from a pharyngeal swab which yielded cytomegalovirus. Complement-fixation, hemagglutination-inhibition, and/or serum-neutralization tests on sequential serum specimens from the patient confirmed that the adenovirus infection occurred during March and showed that infections with cytomegalovirus and respiratory syncytial virus also occurred during late March and April. The patient's persistent fever, for which other causes could not be found, may have been associated with one or more of these infections. Upper respiratory symptoms and lung involvement were not found during this period. Mild liver dysfunction during this time could not be clearly related to adenovirus infection because of the presence of multiple other causes. The adenovirus may have been latent in the donor kidney and become active in the new host as a consequence of immunological impairment. The adenovirus, purified by terminal dilution and plaque procedures, has antigenic, morphological, biophysical, host susceptibility, and hemagglutinating properties characteristic of adenovirus group IA. Buoyant densities in CsCl are 1.340 g/ml for the virion, 1.304 g/ml for the group CF antigen (hexon), 1.295 g/ml for the major soluble complete hemagglutinin (dodecon), and 1.206 g/ml for the minor soluble complete hemagglutinin (tentatively, fiber dimer). The virus does not cross-react in reciprocal hemagglutination-inhibition and serum-neutralization tests with antisera to adenovirus types 1 to 33. We propose this virus as candidate adenovirus type 34 (Compton).

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Selected References

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  1. Armstrong D., Balakrishnan S. L., Steger L., Yu B., Stenzel K. H. Cytomegalovirus infections with viremia following renal transplantation. Arch Intern Med. 1971 Jan;127(1):111–115. [PubMed] [Google Scholar]
  2. Atchison R. W., Casto B. C., Hammon W. M. Electron microscopy of adenovirus-associated virus (AAV) in cell cultures. Virology. 1966 Jun;29(2):353–357. doi: 10.1016/0042-6822(66)90045-6. [DOI] [PubMed] [Google Scholar]
  3. BENYESH-MELNICK M., ROSENBERG H. S. THE ISOLATION OF ADENOVIRUS TYPE 7 FROM A FATAL CASE OF PNEUMONIA AND DISSEMINATED DISEASE. J Pediatr. 1964 Jan;64:83–87. doi: 10.1016/s0022-3476(64)80321-8. [DOI] [PubMed] [Google Scholar]
  4. CHANY C., LEPINE P., LELONG M., LE T. V., SATGE P., VIRAT J. Severe and fatal pneumonia in infants and young children associated with adenovirus infections. Am J Hyg. 1958 May;67(3):367–378. doi: 10.1093/oxfordjournals.aje.a119941. [DOI] [PubMed] [Google Scholar]
  5. Coates H. V., Alling D. W., Chanock R. M. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am J Epidemiol. 1966 Mar;83(2):299–313. doi: 10.1093/oxfordjournals.aje.a120586. [DOI] [PubMed] [Google Scholar]
  6. Collier A. M., Connor J. D., Irving W. R., Jr Generalized type 5 adenovirus infection associated with the pertussis syndrome. J Pediatr. 1966 Dec;69(6):1073–1078. doi: 10.1016/s0022-3476(66)80297-4. [DOI] [PubMed] [Google Scholar]
  7. Dowdle W. R., Lambriex M., Hierholzer J. C. Production and evaluation of a purified adenovirus group-specific (hexon) antigen for use in the diagnostic complement fixation test. Appl Microbiol. 1971 Apr;21(4):718–722. doi: 10.1128/am.21.4.718-722.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GINSBERG H. S. Identification and classification of adenoviruses. Virology. 1962 Oct;18:312–319. doi: 10.1016/0042-6822(62)90018-1. [DOI] [PubMed] [Google Scholar]
  9. GUTEKUNST R. R., HEGGIE A. D. Viremia and viruria in adenovirus infections. Detection in patients with rubella or rubelliform illness. N Engl J Med. 1961 Feb 23;264:374–378. doi: 10.1056/NEJM196102232640803. [DOI] [PubMed] [Google Scholar]
  10. Ginsberg H. S. Adenoviruses. Am J Clin Pathol. 1972 Jun;57(6):771–776. doi: 10.1093/ajcp/57.6.771. [DOI] [PubMed] [Google Scholar]
  11. HANSHAW J. B., WELLER T. H. Urinary excretion of cytomegaloviruses by children with generalized neoplastic disease. Correlation with clinical and histopathologic observations. J Pediatr. 1961 Mar;58:305–311. doi: 10.1016/s0022-3476(61)80259-x. [DOI] [PubMed] [Google Scholar]
  12. Hampil B., Melnick J. L. Method for testing virus stocks for viral contaminants. Appl Microbiol. 1969 Jan;17(1):17–20. doi: 10.1128/am.17.1.17-20.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hierholzer J. C., Barme M. Counterimmunoelectrophoresis with adenovirus type-specific anti-hemagglutinin sera as a rapid diagnostic method. J Immunol. 1974 Mar;112(3):987–995. [PubMed] [Google Scholar]
  14. Hierholzer J. C., Dowdle W. R. Hemagglutination properties of adenovirus types 20, 25, and 28. Proc Soc Exp Biol Med. 1970 Jun;134(2):482–488. doi: 10.3181/00379727-134-34817. [DOI] [PubMed] [Google Scholar]
  15. Hierholzer J. C., Dowdle W. R. Immunological basis of the adenovirus 8-9 cross-reaction. J Virol. 1970 Dec;6(6):782–787. doi: 10.1128/jvi.6.6.782-787.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hierholzer J. C. Further subgrouping of the human adenoviruses by differential hemagglutination. J Infect Dis. 1973 Oct;128(4):541–550. doi: 10.1093/infdis/128.4.541. [DOI] [PubMed] [Google Scholar]
  17. Hierholzer J. C., Gamble W. C., Quist K. D., Chappell W. A. Comparison of immunization methods for producing reference adenovirus antisera in horses. Appl Microbiol. 1972 Sep;24(3):398–404. doi: 10.1128/am.24.3.398-404.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Hierholzer J. C., Pumarola A., Rodriguez-Torres A., Beltran M. Occurrence of respiratory illness due to an atypical strain of adenovirus type 11 during a large outbreak in Spanish military recruits. Am J Epidemiol. 1974 Jun;99(6):434–442. doi: 10.1093/oxfordjournals.aje.a121632. [DOI] [PubMed] [Google Scholar]
  19. Hierholzer J. C., Suggs M. T., Hall E. C. Standardized viral hemagglutination and hemagglutination-inhibition tests. II. Description and statistical evaluation. Appl Microbiol. 1969 Nov;18(5):824–833. doi: 10.1128/am.18.5.824-833.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Hierholzer J. C., Suggs M. T. Standardized viral hemagglutination and hemagglutination-inhibition tests. I. Standardization of erythrocyte suspensions. Appl Microbiol. 1969 Nov;18(5):816–823. doi: 10.1128/am.18.5.816-823.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Hoggan M. D., Blacklow N. R., Rowe W. P. Studies of small DNA viruses found in various adenovirus preparations: physical, biological, and immunological characteristics. Proc Natl Acad Sci U S A. 1966 Jun;55(6):1467–1474. doi: 10.1073/pnas.55.6.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ito M., Mayor H. D. Hemagglutinin of type 4 adeno-associated satellite virus. J Immunol. 1968 Jan;100(1):61–68. [PubMed] [Google Scholar]
  23. Kunin C. M. Viral cystitis. N Engl J Med. 1973 Aug 16;289(7):373–374. doi: 10.1056/NEJM197308162890713. [DOI] [PubMed] [Google Scholar]
  24. Lecatsas G., Prozesky O. W., van Wyk J. Letter: Adenovirus type II associated with haemorrhagic cystitis after renal transplantation. S Afr Med J. 1974 Sep 21;48(46):1932–1932. [PubMed] [Google Scholar]
  25. Manalo D., Mufson M. A., Zollar L. M., Mankad V. N. Adenovirus infection in acute hemorrhagic cystitis. A study in 25 children. Am J Dis Child. 1971 Apr;121(4):281–285. doi: 10.1001/archpedi.1971.02100150055003. [DOI] [PubMed] [Google Scholar]
  26. Mufson M. A., Belshe R. B., Horrigan T. J., Zollar L. M. Cause of acute hemorrhagic cystitis in children. Am J Dis Child. 1973 Nov;126(5):605–609. doi: 10.1001/archpedi.1973.02110190487004. [DOI] [PubMed] [Google Scholar]
  27. Numazaki Y., Kumasaka T., Yano N., Yamanaka M., Miyazawa T., Takai S., Ishida N. Further study on acute hemorrhagic cystitis due to adenovirus type 11. N Engl J Med. 1973 Aug 16;289(7):344–347. doi: 10.1056/NEJM197308162890704. [DOI] [PubMed] [Google Scholar]
  28. Numazaki Y., Shigeta S., Kumasaka T., Miyazawa T., Yamanaka M., Yano N., Takai S., Ishida N. Acute hemorrhagic cystitis in children. Isolation of adenovirus type II. N Engl J Med. 1968 Mar 28;278(13):700–704. doi: 10.1056/NEJM196803282781303. [DOI] [PubMed] [Google Scholar]
  29. Pinkerton H., Carroll S. Fatal adenovirus pneumonia in infants. Correlation of histologic and electron microscopic observations. Am J Pathol. 1971 Dec;65(3):543–548. [PMC free article] [PubMed] [Google Scholar]
  30. ROSEN L. A hemagglutination-inhibition technique for typing adenoviruses. Am J Hyg. 1960 Jan;71:120–128. doi: 10.1093/oxfordjournals.aje.a120085. [DOI] [PubMed] [Google Scholar]
  31. ROWE W. P., HARTLEY J. W., HUEBNER R. J. Additional serotypes of the APC virus group. Proc Soc Exp Biol Med. 1956 Feb;91(2):260–262. doi: 10.3181/00379727-91-22231. [DOI] [PubMed] [Google Scholar]
  32. Rapp F., Melnick J. L. Papovavirus SV40, adenovirus and their hybrids: transformation, complementation, and transcapsidation. Prog Med Virol. 1966;8:349–399. [PubMed] [Google Scholar]
  33. SOHIER R., BENSIMON P., LAGABRIELLE B. Infection à adéno-virus (type 7 prime) avec atteinte pulmonaire et rénale. Bull Mem Soc Med Hop Paris. 1957 Jan 25;73(3):37–42. [PubMed] [Google Scholar]
  34. Stevens D. A., Schaeffer M., Fox J. P., Brandt C. D., Romano M. Standardization and certification of reference antigens and antisera for 30 human adenovirus serotypes. Am J Epidemiol. 1967 Nov;86(3):617–633. doi: 10.1093/oxfordjournals.aje.a120771. [DOI] [PubMed] [Google Scholar]
  35. Tai F. H., Lin H. M., Chu S., Wei H. Y. A new form of acute conjunctivitis epidemic in Taiwan. A simultaneous outbreak of adenovirus type II and "acute hemorrhagic conjunctivitis" virus infections. Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi. 1974 Jun;7(2):79–87. [PubMed] [Google Scholar]
  36. Utz J. P. Viruria in man, an update. Prog Med Virol. 1974;17(0):77–90. [PubMed] [Google Scholar]
  37. VAN DER VEEN J., VAN DER PLOEG G. An outbreak of pharyngoconjunctival fever caused by types 3 and 4 adenovirus at Waalwijk, The Netherlands. Am J Hyg. 1958 Sep;68(2):95–105. doi: 10.1093/oxfordjournals.aje.a119964. [DOI] [PubMed] [Google Scholar]

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