Abstract
A method of indirect immunofluorescence was developed and examined retrospectively as a serological test for the laboratory diagnosis of California encephalitis (CE). LaCrosse virus immunofluorescence immunoglobulin (Ig) G and IgM studies were done on paired sera from 50 patients with acute central nervous system infections. CE had been documented in 25 patients by hemagglutination inhibition, neutralizing, complement fixing, and/or precipitin tests. Five (20%) of the acute and 16 (64%) of the convalescent sera from CE patients had La Crosse IgM antibodies. Seven (28%) of the acute and all of the convalescent CE specimens had La Crosse IgG antibodies. Titers ranged from less than 4 to 256. IgG antibodies were present in all 11 sera collected 1 to 2 years after CE, but IgM antibodies were absent. The 25 serum pairs from patients who did not have CE were negative for IgM and IgG antibodies. This study indicated that La Crosse immunofluorescence antibody tests were as sensitive and specific for CE as conventional hemagglutination-inhibition tests, and would detect at least 20% of patients during their acute illness.
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Selected References
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- Balfour H. H., Jr California (La Crosse virus) encephalitis in Minnesota. Minn Med. 1974 Nov;57(11):876–881. [PubMed] [Google Scholar]
- Balfour H. H., Jr, Edelman C. K. Diagnosis of California (La Crosse) encephalitis by precipitin techniques: a prospective study. Appl Microbiol. 1974 Nov;28(5):807–810. doi: 10.1128/am.28.5.807-810.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Balfour H. H., Jr, Edelman C. K. Letter: Rapid diagnosis of arbovirus infections by counterelectrophoresis. Lancet. 1974 Nov 23;2(7891):1267–1267. doi: 10.1016/s0140-6736(74)90784-3. [DOI] [PubMed] [Google Scholar]
- Balfour H. H., Jr, Majerle R. J., Edelman C. K. California arbovirus (La Crosse) infections. II. Precipitin antibody tests for the diagnosis of California encephalitis. Infect Immun. 1973 Dec;8(6):947–951. doi: 10.1128/iai.8.6.947-951.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Balfour H. H., Jr, Siem R. A., Bauer H., Quie P. G. California arbovirus (La Crosse) infections. I. Clinical and laboratory findings in 66 children with meningoencephalitis. Pediatrics. 1973 Nov;52(5):680–691. [PubMed] [Google Scholar]
- Henderson B. E., Coleman P. H. The growing importance of California arboviruses in the etiology of human disease. Prog Med Virol. 1971;13:404–461. [PubMed] [Google Scholar]
- Henle G., Henle W. Immunofluorescence in cells derived from Burkitt's lymphoma. J Bacteriol. 1966 Mar;91(3):1248–1256. doi: 10.1128/jb.91.3.1248-1256.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]